آثار مکمل جیره‌ای اسیدهای ارگانیک القاء‌کننده ایمنی محافظتی بر علیه کوکسیدیوز

Subject Areas : Camel

آ.ی. عبدوالهی ¹ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China|Department of Animal Science, Kano University of Science and Technology Wudil, PMB3244, Kano, Nigeria)
ایکس.جی. یو ² (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ی.کیو. فو ³ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
م.و. وانگ ⁴ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ن.اس. کی ⁵ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
م.اچ. گیزا ⁶ (College of Animal Science, South China Agricultural University, Guangzhou, China)
اس. کالون ⁷ (Department of Animal Science, Njala University, Freetown, Sierra Leone|College of Animal Science, South China Agricultural University, Guangzhou, China)
و.د. پان ⁸ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ایکس.ل. شی ⁹ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ی. فانگ ¹⁰ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
اس. ویو ¹¹ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
و. هو ¹² (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ل.پی. تان ¹³ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
کا.ایکس. لی ¹⁴ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
ز. وانگ ¹⁵ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)
جی.جی. زو ¹⁶ (College of Animal Science, South China Agricultural University, Guangzhou, China)
د.ی. فنگ ¹⁷ (College of Animal Science, South China Agricultural University, Guangzhou, China)
جی.کیو. لی ¹⁸ (College of Veterinary Medicine, South China Agricultural University, Guangzhou, China)

Received: 2019-02-01 Accepted : 2019-05-15 Published : 2020-03-01

Keywords: ایمنی, اسید ارگانیک, کوکسیدیوزها, سایتوکاین‌ها, سلول T,

Abstract :

این مطالعه آثار سه محصول از اسیدهای ارگانیک جیره‌ای (Acidomix® AFG؛ Activate® AD و Lacplus) روی ایمنی محافظتی بر علیه کوکسیدیوزها را ارزیابی کرد. 280 جوجه 1 روزه گوشتی به ‌طور تصادفی درون 8 گروه شامل 5 تکرار (7 پرنده به ازای هر تکرار)، شامل 2 کنترل (منفی=N؛ مثبت=P) و 6 گروه مکمل اسید ارگانیک (1 درصد AFG، 1 درصد DA، 1 درصد Lacplus، 2 درصد AFG، 2 درصد DA و 2 درصد Lacplus) تقسیم شدند. به استثنای کنترل N (آلوده نشده) ، تمامی 7 گروه به ‌طور خوراکی با 105 × 8 از اووسیت‌های هاگ‌آور شده Eimeria tenella در روز 12 بعد از هچ به چالش کشیده شدند. نمونه‌های خون، طحال و روده کور در روز 22 و 35 بعد از هچ جمع‌آوری شدند. رشد وزنی بدن، خارج شدن اووسیت مدفوعی، درجه تخریب، نرخ مرگ و میر و pH سکوم بررسی شدند. تست ELISA برای تشخیص خوشه مولکول‌های سلول‌ T افتراقی (CD4+ و CD8+) استفاده شده و واکنش زنجیره‌ای پلیمراز رونوشت‌بردار معکوس (RT-PCR) برای تشخیص کموکاین‌های پیش التهابی (INFγ) و بیان سایتوکاین‌ها (IL-8، IL-15 و IL-17) بکار گرفته شدند. نتیجه نشان داد که مکمل‌سازی اسیدهای ارگانیک به ‌طور معنی‌داری رشد وزنی بدن را افزایش، ضریب تبدیل خوراک را بهبود، و درجات جراحات و شاخص اووسیت (0.05>P) در مقایسه با گروه کنترل P (آلوده شده غیر مکمل) را کاهش داد. نرخ مرگ و میر در گروه کنترل P بالاتر بود. pH روده کور معنی‌دار بود (0.05>P) و حداکثر در گروه کنترل P مشاهده شد. جوجه‌های تغذیه شده با 2 درصد DA و 1 درصد Lacplus درصد بالاتری از مولکول‌های سلول T CD4 داشتند، اما مولکول‌های سلول T CD8 کمتری داشتند، که ارائه کننده یک عملکرد محافظتی از این زیرمجموعه‌های سلول T در پاسخ ایمنی ذاتی در مقابل عفونت E. tenella است. کموکاین‌های روده کور و طحال و بیان mRNA سایتوکاین‌ها شامل INFγ، IL-8، IL-15 و IL-17 سطوح بالاتری از رونویسی در مقایسه با کنترل‌های N و P نشان دادند، که نشان می‌دهد محصولات اسید ارگانیک ممکن است با بهبود تولید آنها آثار محافظتی خود را اعمال کرده باشند. نتیجه گرفته شده که Activate® DA در سطوح 2 درصد و Laxplus در سطوح 1 درصد هم در روز 22 و هم در روز 35 بعد از هچ بهترین آثار ضدکوکسیدیال را نشان داده‌اند.

References:

Abbas R.Z., Iqbal Z., Khan M.N., Zafar M.A. and Zia M.A. (2010). Anticoccidial activity of Curcuma longa in broiler chickens. Braz. Arch. Biol. Technol. 53, 63-67.

Abbas R.Z., Munawar S.H., Manzoor Z., Iqbal Z., Khan M.N., Saleemi M.K., Zia M.A. and Yousaf A. (2011). Anticoccidial effects of acetic acid on performance and pathogenic parameters in broiler chickens challenged with Eimeria tenella. Pesq. Vet. Bras. 31, 99-103.

Abudabos A.M. and Al-Mufarrej S.I. (2014). Effects of organic acid supplementation on antioxidant capacity and immune responses of broilers challenged orally with Salmonella enterica subsp. enterica Typhimurium. South African J. Anim. Sci. 44, 360-370.

Abudabos A.M., Alyemni A.H., Dafalla Y.M. and Khan R.U. (2017). Effect of organic acid blend and Bacillus subtilis alone or in combination on growth traits, blood biochemical and antioxidant status in broilers exposed to Salmonella typhimurium challenge during the starter phase. J. Appl. Anim. Res. 45, 538-542.

Alhidary I.A., Abdelrahman M.M. and Khan R.U. (2016). Comparative effects of direct-fed microbial alone or with a traces mineral supplement on the productive performance, blood metabolites and antioxidant status of grazing Awassi lambs. Environ. Sci. Pollut. Res. 23, 25218-25223.

Blake D.P. and Tomley F.M. (2014). Securing poultry production from the ever-present Eimeria challenge. Trends Parasitol. 30, 12-19.

Carriere D., Fountain C., Berthier A.M., Rouquette A.M., Carayon P., Laprode M., Juillard R., Jansen A., Paoli P., Paolucci F., Gros P. and Pau B. (1994). Two-site enzyme immunoassay of CD4 and CD8 molecules on the surface of T lymphocytes from healthy subjects and HIV infected patient. Clin. Chem. 40, 30-37.

Çilek S. and Tekin M.E. (2005). The environmental factors affecting milk yield and fertility traits of simmental cattle raised at the Kazova State farm and phenotypic correlations between these traits. Turkish J. Vet. Anim. Sci. 29, 987-993.

Cuckler A.C. (1959). The laboratory evaluation of coccidiostatic drugs. Pp. 1-14 in Proc. Conf. Methods. Test. Coccidistats. Merek Chem. Divison. Rahway, New Jersey. 

Dalloul R.A. and Lillehoj H.S. (2006). Poultry coccidiosis: Recent advancements in control measures and vaccine development. Exp. Rev. Vac. 5, 143-163.

Dalloul R.A., Lillehoj H.S., Klinman D.M., Ding X., Min W., Heckert R.A. and Lillehoj E.P. (2005). In ovo administration of CpG oligodeoxynucleotides and the recombinant microneme protein MIC2 protects against Eimeria infections. Vaccine. 23, 3108-3113.

Dana K.D., Steven C.R. and Aaron S.K. (2018). Organic acids and potential for modifying the avian gastrointestinal tract and reducing pathogens and disease. Front. Vet. Sci. 5, 216-222.

De Gussem M. (2007). Coccidiosis in poultry: Review on diagnosis, control, prevention and interaction with overall gut health. Pp. 45-52 in Proc. 16^th European Symp. Poult. Nutr., Strasbourg, France.

Dhama K., Tiwari R., Khan R.U., Chakroborty S., Gopi M., Kathik K., Saminathan M., Desingu P.A. and Sunkara L.T. (2014). Growth promoters and novel feed additives improving poultry production and health, bioactive principles and beneficial applications: the trends and advances-a review. Int. J. Pharmacol. 10, 129-159.

Dibner J.J. and Buttin P. (2002). Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. J. Appl. Poult. Res. 11, 453-463.

Du A. and Hu S. (2004). Effects of a herbal complex against Eimeria tenella infection in chickens. J. Vet. Med. 51, 194-197.

Fathi R., Saleh-Samadi M., Qotbi A.A.A., Seidavi A.R. and Martínez Marín A.L. (2016). Effects of feed supplementation with increasing levels of organic acids on growth performance, carcass traits, gut microbiota and pH, plasma metabolites, and immune response of broilers. Anim. Sci. Pap. Rep. 34(2), 195-206.

Ghazvinian K., Seidavi A.R., Laudadio F., Ragni M. and Tufarelli V. (2018). Effects of various levels of organic acids and of virginiamycin on performance, blood parameters, immunoglobulins and microbial population of broiler chicks. South African J. Anim. Sci. 48(5), 961-967.

Hong Y.H., Lillehoj H.S., Lee S.H., Dalloul R.A. and Lillehoj E.P. (2006a). Analysis of chicken cytokine and chemokine gene expression following and infections. Vet. Immunol. Immunopathol. 114, 209-223.

Hong Y.H., Lillehoj H.S., Lillehoj E.P. and Lee S.H. (2006b). Changes in immune-related gene expression and intestinal lymphocyte subpopulations following infection of chickens. Vet. Immunol. Immunopathol. 114, 257-272.

Idris A.B., Bounous D.I., Goodwin M.A., Brown J. and Krushinskie E.A. (1997). Lack of correlation between microscopic lesion scores and gross lesion scores in commercially grown broilers examined for small intestinal Eimeria spp. coccidiosis. Avian Dis. 41, 388-391.

Johnson J. and Reid W.M. (1970). Anticoccidial drugs: Lesion scoring techniques in battery and floor pen experiments with chickens. Exp. Parasitol. 28, 30-36.

Khan R.U., Naz S., Nikousefat Z., Tufarelli V., Javdani M., Qureshi M.S. and Laudadio V. (2012). Potential applications of ginger (Zingiber officinale) in poultry diets. World Poult. Sci. J. 68, 245-252.

Khan R.U., Chand N. and Ali A. (2016a). Effect of organic acids on the performance of Japanese quails. Pakistan J. Zool. 48, 1799-1803.

Lee S.H., Lillehoj H., Lillehoj E.P., Cho S.M., Park D.W., Hong Y.H., Chun H.K. and Park H.J. (2008). Immunomudulatory properties of dietary plum on coccidiosis. Compar. Immunol. Microbiol. Infect. Dis. 31, 389-402.

Lee S.H., Lillehoj H.S., Dalloul R.A., Park D.W., Hong Y.H. and Lin J.J. (2007a). Influence of Pediococcus-based probiotic on coccidiosis in broiler chickens. Poult. Sci. 86, 63-46.

Lee S.H., Lillehoj H.S., Park D.W., Hong Y.H. and Lin J.J. (2007b). Effect of Pediococcus and Saccharomyces-based probiotic (MitoMaxs) on coccidiosis in broiler chickens. Comp. Immunol. Microbiol. 30, 261-268.

Lee S.H., Lillehoj H.S., Park D.W., Hong Y.H., Cho S.M., Chun H.K. and Park H.J. (2007c). Immunomudulatory effect of dietary safflower leaf in the chicken. Korean J. Commun. Living Sci. 18, 214-224.

Lee S.H., Lillehoj H.S., Cho S.M., Park D.W., Hong Y.H., Lillehoj E.P., Heckert R.A., Park H.J. and Chun H.K. (2009). Protective effects of dietary safflower (Carthamus tinctorius) on experimental coccidiosis. J. Poult. Sci. 46, 155-162.

Lillehoj H.S. and Choi K.D. (1998). Recombinant chicken interferon- mediated inhibition of development and reduction of oocyst production and body weight loss following challenge infection. Avian Dis. 42, 307-314.

Lillehoj H.S., Min W., Choi K.D., Babu U.S., Burnside J., Miyamoto T., Rosenthal B.M. and Lillehoj E.P. (2001). Molecular, cellular, and functional characterization of chicken cytokines homologous to mammalian IL-15 and IL-2. Vet. Immunol. Immunopathol. 82, 229-244.

Lillehoj H.S., Min W.G. and Dalloul R.A. (2004). Recent progress on the cytokine regulation of intestinal immune response to Eimeria. Poult. Sci. 83, 611-623.

Livak K.J. and Schmittgen T.D. (2001). Analysis of relative gene expression data using real time quantitative PCR and the 2^-∆∆CT method. Methods. 25, 402-408,

Lückstädt C. and Kühlmann K. (2013). Use of drinking water acidification to enhance poultry performance in rural Thailand. Pp. 21-31 in Proc. Conf. Int. Res. Food Secur., Stuttgart, Germany.

Lutz F., Elsa N., WoIf-Dietrich D. and Tomas P. (1994). Quantitative determination of CD4/CD8 molecules by a cell marker ELISA. Clin. Chem. 40, 38-42.

Mahanta J.D., Borgohain B., Sharma M., Sapcota D. and Hussain, J. (2017). Effect of dietary supplementation of herbal growth promoter on performance of commercial broiler chickens. Indian J. Anim. Res. 51, 1097-1100.

Manzoor Z., Munawara S.H., Abbasa R.Z., Razab M.A. and Khan I.A. (2013). Comparative anticoccidial activity of hydrochloric acid (HCl) and formic acid against Eimeria tenella in broiler chickens. Int. J. Biol. Med. Res. 4, 3714-3718.

McSorley S.J., Cookson B.T. and Jenkins M.K. (2000). Characterization of CD4⁺ T-cell responses during natural infection with Salmonella typhimurium. J. Immunol. 164, 986-993.

Nicholson J.K.A., Velleca W.M., Jubert S., Green T.A. and Bryan L. (1994). Evaluation of alternative CD4 technology for the enumeration of lymphocytes. J. Immunol. Methods. 177, 43-54.

Okoro V.M.O., Nwokeocha A.C.C., Ijezie C.O., Mbajiorgu C.A. and Mbajiorgu E.F. (2016). Effect of varying dietary supplemental inclusion levels of onion and garlic on semen quality characteristics of Hubbard white breeder broiler cocks aged 35-41 weeks old. Indian J. Anim. Res. 50, 922-929.

Pinilla Moraza J., Anton Botella F., Labarga Echeverria P., Sadaba Selbado C., Gimeno VillaR C. and Mugica Vargas M. (1997). Course and prognosis of levels of CD4 molecules in HIV patients. Anal. Med. Interna. 14, 345-347.

Richard Sygall D.V.M. (2003). The effect of organic acids on chickens infected with Eimeria. Int. Poult. Prod. 20(3), 17-19.

Serge D., Georges D., Paul Thomas S., Honorine D. and Eric L. (1999). Evaluation of a quantitative determination of CD4 and CD8 molecules as an alternative to CD4⁺ and CD8⁺ T-lymphocytes in Africans. Trop. Med. Int. Health. 4, 79-84.

Sharman P.A., Smith N.C., Wallach M.G. and Katrib M. (2010). Chasing the golden egg: Vaccination against poultry coccidiosis. Parasite Immunol. 32, 590-598.

Williams R.B. (2005). Intercurrent coccidiosis and necrotic enteritis of chickens: rational, integrated disease management by maintenance of gut integrity. Avian Pathol. 34, 159-180.

World Health Organization. (1992). Workshop on flow cytometry and alternative methodologies for CD4 lymphocytes determinations: application for developing countries. Washington D.C., 1992. World Health Organization, Geneva.

Yun C.H., Estrada A., Kessel A.V., Park B.C. and Laarveld B. (2003). β-glucan, extracted from oats, enhances disease against bacterial and parasitic infections. FEMS Immunol. Med. Microbiol. 35, 67-75.