ارتباط چندشکلی‌های ژن IGF-I با صفات لاشه در گوسفندان نژاد مهربان با استفاده از روش SSCP

Subject Areas : Camel

S. Behzadi ¹ (Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran)
S.R. Miraei-Ashtiani ² (Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran)
M. Sadeghi ³ (Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran)
P. Zamani ⁴ (Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran)
R. Abdoli ⁵ (Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran)

Received: 2014-01-08 Accepted : 2014-04-30 Published : 2015-03-01

Keywords: صفات لاشه, فاکتور رشد شبه انسولین نوع 1, گوسفندان مهربان, چندشکلی‌های تک نوکلئوتیدی, چندشکلی آرایش فضایی رشته‌های منفرد,

Abstract :

انتخاب به کمک ژنتیک مولکولی با استفاده از ژن­های اختصاصی یک روش پیشنهاد شده برای بهبود ژنتیکی صفات مهم اقتصادی در دام‌ها می­باشد. ژن IGF-I ممکن است نقش­های مهمی در رشد بافت­های متفاوت شامل سلول­های عضلانی، غضروف و استخوان بازی کند. اهداف مطالعه حاضر برآورد فراوانی­های هاپلوتایپی چندشکلی­های ژن IGF-I در گوسفندان نژاد مهربان و تعیین ارتباط بین این چندشکلی­ها و صفات لاشه در 439 حیوان بودند. واکنش زنجیره­ای پلیمراز با استفاده از روش­های چندشکلی­ آرایش فضایی رشته‌های منفرد (PCR-SSCP) و توالی یابی برای شناسایی تفاوت­های ژنتیکی بکار گرفته شدند. دو چندشکلی تک نوکلئوتیدی (C114G و G116A) و دو تغییر آمینو اسیدی (S5T و G6S) با سه الگوی متفاوت در قطعه تکثیر شده از ژن IGF-I شناسایی شدند. ارتباط الگوهای ژن IGF-I با کلسترول و تریگلیسرید خون معنی­دار (05/0P<) و برای ضخامت چربی پشتی و وزن لاشه متمایل به معنی­دار (1/0P<) بود. دیگر صفات مطالعه شده تحت تأثیر معنی‌داری به وسیله الگوهای متفاوت ژن IGF-I قرار نگرفتند. این یافته­ها نشان می­دهند که چندشکلی­های ژن IGF-I ممکن است به عنوان نشانگرهای DNA برای انتخاب در فرآیندهای اصلاح نژادی در گوسفندان نژاد مهربان مورد استفاده قرار گیرند.

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