تأثیر تزریق درون تخم مرغی استات روی بر بیان برخی از ژن های مرتبط با رشد و نمو جنینی و برخی از ویژگی های لاشه جوجه ها

Subject Areas : Camel

م. تقی پور شه بندی ¹ , ز. انصاری پیرسرائی ² , ح. دلدار ³ , م. کاظمی فرد ⁴

1 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
4 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran

Received: 2017-04-18 Accepted : 2017-07-15 Published : 2019-03-01

Keywords: روی, رشد و نمو, جوجه ها, تزریق درون تخم مرغی, فاکتورهای رشد انسولین مانند, فاکتورهای تنظیمی ماهیچه,

Abstract :

در این پژوهش تأثیر تزریق­ درون تخم­ مرغی استات روی (ZAC)، بر بیان برخی از ژن ­های مرتبط با رشد و نمو جنینی و برخی از ویژگی ­های لاشه جوجه ­ها طی دو گامه بررسی شد. درگامه نخست تأثیر تزریق­ درون تخم ­مرغی ZAC، بر بیان نسبی ژن ­های فاکتورهای رشد انسولین مانند (IGF-I، IGF-II و IGF-IR)، فاکتورهای تنظیمی ماهیچه (MyoD و MYf-5) و متالوتیونین (MT) بررسی، و در گامه دوم ارتباط بین بیان ژن­ ها با باروری، مرگ و میر جنینی، جوجه ­­دهی، وزن بدن (BW)، درصد وزن اجزای لاشه در روز تولد، وزن بدن (BW) و ضریب تبدیل غذایی (FCR) هفت روز پس از تولد بررسی شد. هفتصد و بیست تخم ­مرغ بارور (n=720) به طور تصادفی به چهار تیمار تقسیم شدند که در برگیرنده­: 1) بدون تزریق(NI)، 2) تزریق آب بدون یون (DW)، 3) و 4) به ترتیب تزریق 3.64 و 5.23 مولار استات روی (به ­ترتیب ZAC 3.64  و ZAC 5.23) بود. در روز سه جوجه­ کشی، مقدار 50 میکرولیتر از هر تیمار در آلبومن تزریق شد. در روز پنج جوجه­ کشی و در روز تولد بافت جگر و ماهیچه سینه جوجه­ ها جدا شدند و فوری در 80- درجه سانتیگراد تا روز استخراج RNA و آنالیز real time-PCR ذخیره شدند. روی باعث افزایش بیان فاکتور تمایز مایوژنیک (MyoD) در بافت جنینی و ماهیچه شد. بیان mRNAی MT با تزریق ZAC افزایش یافت. یا درصد جوجه ­دهی در تیمارهای تزریق استات روی کمترین و مرگ‌ و‌ میر در گامه نخست جوجه ­کشی در تیمارهای ZAC و SHC (sham control) بیشترین بود. تزریق ZAC باعث بهبود BW و FCR در هفته نخست پرورش و افزایش درصد سینه و جگر شد.در نتیجه تزریق ZAC سبب افزایش بیان نسبی برخی از ژن­ های در برخی از بافت ­ها و BW7 طی هفته نخست شد اما درصد جوجه­ دهی کاهش یافت. بنابراین پیشنهاد می ­شود که در پژوهش ­های آینده از دوزهای کمتر استات­ روی استفاده شود.

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