نمایی از چندشکلی های تک نوکلئوتیدی در ژن های باروری در میان نژادهای گوسفند ایران با بکارگیری روش PCR-RFLP

Subject Areas : Camel

پ. پتکی ¹ (Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran)
س.ض. میرحسینی ² (Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)
ف. افراز ³ (Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran)
س.م.ف. وحیدی ⁴ (Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran)

Received: 2019-01-14 Accepted : 2019-08-15 Published : 2020-06-01

Keywords: PCR-RFLP, GDF9, گوسفند, BMP15, BMPR1B, جهش تک نوکلئوتیدی,

Abstract :

نرخ تخمک­ گذاری و میزان بره ­زایی، از ویژگی­های باروری بوده که ارزش اقتصادی فراوانی در گوسفندان داشته و به افزایش تولید گوشت، پشم و شیر می­ انجامد. از این رو کاوش بر روی ژن­ های کاندید و کارساز بر این ویژگی­ ها جایگاه ارزنده­ای دارد. سه ژن­ GDF9، BMP15 و BMPR1B از مهم­ترین این ژن­ ها هستند که دارای چندین جهش تک­نوکلئوتیدی (SNPs) مرتبط با باروری بالا در گوسفندان می ­باشند. در این پژوهش، به برآورد فراوانی ژنی و ژنوتیپی هفت جهش تک نوکلئوتیدی؛ جهش­ های G1، G4، G7 و G8 در ژن GDF9، جهش های B2 و B4 در ژن  BMP15 و جهش FecB در ژن BMPR1B در میان 532 گوسفند از 11 نژاد گوسفندان بومی ایران به روش PCR-RFLP پرداخته شد. به ازای هر یک از مناطق جهشی، واکنش­ های زنجیره­ای پلی­مراز و نیز گوارش آنزیمی با آنزیم­ های برشی ویژه انجام شد. دستاوردها نشان داد که جهش G1 در ژن GDF9 در میان 10 نژاد بجز نژاد زندی، جهش G4 در همین ژن در میان هر 11 نژاد و جهش FecB در ژن BMPR1B تنها در میان دو نژاد کلکویی و زندی وجود دارد. جهش ­های G7 و G8 در ژن GDF9 و جهش­ های B2 و B4 در ژن  BMP15 در هیچ یک از این 11 نژاد دیده نشدند و همگی گوسفندان برای این چهار جایگاه، هموزیگوت غالب بودند.

References:

Abraham A. and Thomas N. (2012). Role of fecundity genes in prolificacy of small ruminants. J. Ind. Vet. Assoc. Kerala. 10(3), 34-37.

Agricultural Statistics of Iran. (2017). Ministry of Jihad-e-Agriculture, Deputy Director of Planning and Economics. Center for Information and Communication Technology. Tehran, Iran.

Ahmadi A., Afraz F., Talebi R., Farahavar A. and Vahidi S.M.F. (2016). Investigation of GDF9 and BMP15 polymorphisms in Mehraban sheep to find the missenses as impact on protein. Iranian J. Appl. Anim. Sci. 6(4), 863-872.

Akbarinejad V., Kazempooor R., Shojaei M., Rezaee M. and Oji R. (2014). Atlas of Iranian Sheep Breeds. Noorbakhsh Press upon the Request of Biotechnology Development Council. Tehran, Iran.

Akbarpour M., Houshmand M., Ghorashi A. and Hayatgheibi H. (2008). Screening for FecG^H mutation of growth differentiation factor 9 gene in Iranian Ghezel sheep population. Royan Inst. Int. J. Fertil. Steril. 2(3), 139-144.

Al-Barzinji Y.M. and Othman G.U. (2013). Genetic polymorphism in FecB gene in Iraqi sheep breeds using RFLP-PCR technique. IOSR J. Agric. Vet. Sci. 2(4), 46-48.

Ali A.S., Ibrahim M.T., Mohammed M.M., Elobied A.A. and Lühken G. (2016). Growth differentiation factor 9 gene variants in Sudanese desert sheep ecotypes. South African J. Anim. Sci. 46(4), 373-379.

Bahrami Y., Bahrami S., Mohammadi H.R., Chekani-Azar V. and Mousavizadeh S.A. (2014). The polymorphism of GDF-9 gene in Hisari sheep. Biol. Forum. 6(2), 46-52.

Chu M., Jia L., Zhang Y., Jin M., Chen H., Fang L., Di R., Cao G., Feng T., Tang Q., Ma Y. and Li K. (2011). Polymorphisms of coding region of BMPR-IB gene and their relationship with litter size in sheep. Mol. Biol. Rep. 38(6), 4071-4076.

Chu M.X., Liu Z.H., Jiao C.L., He Y.Q., Fang L., Ye S.C., Chen G.H. and Wang J.Y. (2006). Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries). J. Anim. Scence. 85, 598-603.

Dash S., Maity A., Bisoi P.C., Palai T.K., Polley S., Mukherjee A. and De S. (2017). Coexistance of polymorphism in fecundity genes BMPR1B and GDF9 of Indian Kendrapada sheep. Exp. Anim. Med. Res. 7(1), 33-38.

Davis G.H., Farquhar P.A., O'Connell A.R., Everett-Hincks J.M., Wishart P.J., Galloway S.M. and Dodds K.G. (2006). A putative autosomal gene increasing ovulation rate in Romney sheep. Anim. Reprod. Sci. 92(1), 65-73.

Davis G.H., Galloway S.M., Ross K.I., Gergan S.M., Ward J., Nimbkar B.V., Ghalsasi P.M., Nimbkar C., Gray G.D., Inounu I., Tiesnamurti B., Hanrahan J.P. and Wilson T. (2002). DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biol. Reprod. 66, 1869-1874.

Debnath J. and Singh R.V. (2014). Genetic polymorphism of boorola FecB gene and its association with litter size in Balangir, Shahabadi and Bonpala sheep breeds. Indian J. Anim. Res. 48(4), 307-314.

Dincel D., Ardicli S., Samli H. and Balci F. (2018). Genotype frequency of FecX^B (Belclare) mutation of BMP15 gene in Chios (Sakiz) sheep. Uludag University J. Fac. Vet. Med. 37(2), 1-5.

Eghbalsaied S., Amini H., Shahmoradi S. and Farahi M. (2014). Simultaneous presence of G1 and G4 mutations in growth differentiation factor 9 gene of Iranian sheep. Iranian J. Appl. Anim. Sci. 4(4), 781-785.

El-Hanafy A.A. and El-Saadani M.A. (2009). Fingerprinting of FecB gene in five Egyptian sheep breeds. Biotechnol. Anim. Husb. 25(3), 205-212.

Escobar-Chaparro R.A., Guillén G., Espejo-Galicia L.U., Meza-Villalvazo V.M., Pena-Castro J.M. and Abad-Zavaleta J. (2017). qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy. 3 Biotech. 7(3), 204-211.

Esen Gursel F., Akis I., Durak H., Mengi A. and Oztabak K. (2011). Determination of BMP-15, BMPR-1B and GDF-9 gene mutations of the indigenous sheep breeds in Turkey. Kafkas Univ. Vet. Fak. Derg. 17(5), 725-729.

Galloway S.M., McNatty K.P., Cambridge L.M., Laitinen M.P., Juengel J.L., Jokiranta T.S., McLaren R.J., Luiro K., Dodds K.G., Montgomery G.W., Beattie A.E., Davis G.H. and Ritvos O. (2000). Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nat. Genet. 25(3), 279-283.

Ghaderi A., Beigi nasiri M.T., Mirzadeh K.H., Fayazi J. and Sadr A.S. (2010). Identification of the GDF9 mutation in two sheep breeds by using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) technique. African J. Biotechnol. 9(47), 8020-8022.

Hanrahan J.P., Gregan S.M., Mulsant P., Mullen M., Davis G.H., Powell R. and Galloway S.M. (2004). Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol. Reprod. 70, 900-909.

Holanda G.M.L., Oliveira J.C., Silva D.M.F., Rocha S.S.N., Pandolfi V., Adriao M. and Wischral A. (2017). Survey of mutations in prolificacy genes in Santa Ines and Morada Nova sheep. Arq. Bras. Med. Vet. Zootec. 69(4), 1047-1053.

Jamshidi R., Kasirian M.M. and Rahimi G.A. (2013). Application of PCR-RFLP technique to determine Booroola gene polymorphism in the Sangsari sheep breed of Iran. Turkish J. Vet. Anim. Sci. 37(2), 129-133.

Jansson T. (2014). Genes involved in ovulation rate and litter size in sheep. BScThesis. Examensarbete, Swedish University of Agricultural Science, Uppsala, Sweden.

Jawasreh K.I.Z., Awawdeh F.T. and Al-Qaisy A. (2014). Association between GDF9, FecB and prolactin gene polymorphisms and prolificacy of Awassi sheep. Pp. 25-32 in 10^th World Congr. Genet. Appl. Livest. Prod. Vancouver, Canada.

Jia C., Li N., Zhao X., Zhu X. and Jia Z. (2005). Association of single nucleotide polymorphisms in exon 6 region of BMPRIB gene with litter size traits in sheep. Asian Australian J. Anim. Sci. 18(10), 1375-1378.

Karsli T., Sahin E., Karsli B.A., Alkan S. and Balcioglu M.S. (2012). An investigation of mutations (FecX^G, FecX^I, FecX^H, FecX^B) on BMP-15 gene in some local sheep breeds raised in Turkey. Akdeniz Üniv. Ziraat Fak. 25(1), 29-33.

Khaldari M. (2014). Sheep and Goat Husbandry. Publishing Organization, Tehran, Iran.

Khanahmadi A., Gharehveysi S., Khatamnejad R. and Arab J. (2014). Polymorphic variants of G1 and B4 from GDF9 and BMP15 genes of Dalagh sheep. Res. Anim. Prod. 5(10), 148-155.

Khatamnejhad R. and Khanahmadi A. (2012). Genetic polymorphism in GDF9 and FecB genes in Dalagh sheep breed of Iran. J. Anim. Vet. Adv. 11(6), 766-768.

Khodabakhshzadeh R., Mohammadabadi M.R., Esmailizadeh A.K., Shahrebabak H.M., Bordbar F. and Namin S.A. (2016). Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Polish J. Vet. Sci. 19(2), 281-289.

Kumar R., Taraphder S., Sahoo A., Senpati P., Paul R., Roy M., Sarkar U., Raja D., Samanta I. and Baidya S. (2016). Polymorphism and nucleotide sequencing of BMP-15 gene in an organised herd of Garole sheep (Ovis aries). Indian J. Anim. Hlth. 55(1), 77-86.

Kumar S., Mishra A.K., Kolte A.P., Dash S.K. and Karim S.A. (2008). Screening for Booroola (FecB) and Galway (FecX^G) mutations in Indian sheep. Small Rumin. Res. 80(1), 57-61.

Liandris E., Kominakis A., Andreadou M., Kapeoldassi K., Chadio S., Tsiligianni T., Gazouli M. and Ikonomopoulos I. (2012). Associations between single nucleotide polymorphisms of GDF9 and BMP15 genes and litter size in two dairy sheep breeds of Greece. Small Rumin. Res. 107, 16-21.

Lopez-Ramirez R.B., Magana-Sevilla H.F., Zamora-Bustillos R., Ramon-Ugalde J.P. and Gonzalez-Mendoza D. (2014). Analysis of the 3′ end regions of the GDF9 and BMPR1B genes in Blackbelly sheep from Yucatán, Mexico. Cien. Inv. Agr. 41(1), 123-128.

Mahdavi M., Nanekarani S. and Hosseini S.D. (2014). Mutation in BMPR-IB gene is associated with litter size in Iranian Kalehkoohi sheep. Anim. Reprod. Sci. 147(3), 93-98.

Mahrous K.F., Hassanane M.S., Abdel Mordy M., Shafey H.I. and Rushdi H.E. (2015). Polymorphism of some genes associated with meat-related traits in Egyptian sheep breeds. Iranian J. Appl. Anim. Sci. 5(3), 655-663.

Maskur M., Tapaul R. and Kasip L. (2016). Genetic polymorphism of bone morphogenetic protein receptor 1B (BMPR-1B) gene and its association with litter size in Indonesian fat-Tailed sheep. African J. Biotechnol. 15(25), 1315-1319.

Moradband F., Rahimi G. and Gholizadeh M. (2011). Association of polymorphisms in fecundity genes of GDF9, BMP15 and BMP15-1B with litter size in Iranian Baluchi sheep. Asian-Australasian J. Anim. Sci. 24(9), 1179-1183.

Mullen M.P. and Hanrahan J.P. (2014). Direct evidence on the contribution of a missense mutation in GDF9 to variation in ovulation rate of Finnsheep. PloS One. 9(4), e95251.

Mullen M.P., Hanrahan J.P., Howard D.J. and Powell R. (2013). Investigation of prolific sheep from UK and Ireland for evidence on origin of the mutations in BMP15 (FecX^G, FecX^B) and GDF9 (FecG^H) in Belclare and Cambridge sheep. PloS One. 8(1), e53172.

Mulsant P., Lecerf F., Fabre S., Schibler L., Monget P., Lanneluc I., Pisselet C., Riquet J., Monniaux D., Callebaut I., Cribiu E., Thimonier J., Teyssier J., Bodin L., Cognié Y., Chitour N. and Elsen J.M. (2001). Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes. Proc. Natl. Acad. Sci. 98(9), 5104-5109.

Nagdy H., Mahmoud K.G.M., Kandiel M.M., Helmy N.A., Ibrahim S.S., Nawito M.F. and Othman O.E. (2018). PCR-RFLP of bone morphogenetic protein 15 (BMP15/FecX) gene as a candidate for prolificacy in sheep. Int. J. Vet. Sci. Med. 6, 68-72.

Nanekarani S., Goodarzi M., Khederzadeh S., Torabi S. and Landy N. (2016). Detection of polymorphism in Booroola gene and growth differentiation factor 9 in Lori sheep breed. Trop. J. Pharm. Res. 15(8), 1605-1611.

Oraon T., Singh D.K., Ghosh M., Kullu S.S., Kumar R. and Singh L.B. (2016). Allelic and genotypic frequencies in polymorphic Booroola fecundity gene and their association with multiple birth and postnatal growth in Chhotanagpuri sheep. Vet. World. 9(11), 1294-1299.

Pan Z., Zhang B., Hu W., Di R., Lin Q., Wang X., Yin D., Wang P. and Chu M. (2015). The genetic diversity of both FecB gene and microsatellite GC101 is associated with reproduction selection in sheep. Turkish J. Vet. Anim. Sci. 39(3), 254-260.

Paz E., Quinones J., Bravo S., Montaldo H.H. and Sepulveda N. (2015). Genotyping of BMPR1B, BMP15 and GDF9 genes in Chilean sheep breeds and association with prolificacy. Anim. Genet. 46(1), 98-109.

Paz E., Quinones J., Bravo S., Rodero E., Gonzalez A. and Sepulveda N. (2014). Identification of G1 and G8 polymorphisms of GDF9 gene in Araucano creole sheep. Arch. Med. Vet. 46, 327-331.

Pirzadi Y., Nanakarani S. and Godarzi M. (2014). Genetic polymorphism of GDF9 gene in Lori-Romanov crossbreeds sheep by PCR-RFLP. Pp. 45-51 in 1^st Int. Conf. New Ideas. Agric. Islamic Azad University Khorasgan Branch, Isfahan, Iran.

Polley S., De S., Brahma B., Mukherjee A., Vinesh P.V., Batabyal S., Arora J.S., Pan S., Samanta A.K., Datta T.K. and Goswami S.L. (2010). Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep. Trop. Anim. Health Prod. 42, 985-993.

Qiuyue L., Zhangyuan P., Xiangyu W., Wenping H., Ran D., Yaxing Y. and Mingxing C. (2014). Progress on major genes for high fecundity in ewes. Front. Agric. Sci. Engin. 1(4), 282-290.

Roy J., Polley S., De S., Mukherjee A., Batabyal S., Pan S., Brahma B., Datta T.K. and Goswami S.L. (2011). Polymorphism of fecundity genes (FecB, FecX and FecG) in the Indian Bonpala sheep. Anim. Biotechnol. 22(3), 151-162.

Saadatnouri M. and Siahmansour S. (2011). Principles of Sheep Husbandry. Ashrafi Publications, Tehran, Iran.

Shafieiyan Z., Mohammadi G., Jolodarzadeh A. Amiri S. (2013). No mutations of FecB and FecG^H in Iranian Lory sheep. Vet. Res. Forum. 4(4), 265-268.

Shi H., Bai J., Niu Z., Muniresha Fen L. and Jia B. (2010). Study on candidate gene for fecundity traits in xingjiang cele black sheep. African J. Biotechnol. 9(49), 8498-8505.

Siddiqua S.A. (2013). Phenotypic effects of a fertility mutation in Norwgain White sheep. MS Thesis. Norwegian University of Life Science, Ås, Norway.

Somarny W.W., Erin A.R., Suhaimi A.H.M.S., Nurulhuda M.O. and Hifzan R.M. (2013). A study of major prolificacy genes in Malin and Dorper sheep in Malaysia. J. Trop. Agric. Food Sci. 41(2), 265-272.

Souza C.J.H., McNeilly A.S., Benavides M.V., Melo E.O. and Moraes J.C.F. (2014). Mutation in the protease cleavage site of GDF 9 increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes. Anim. Genet. 45(5), 732-739.

Sudhakar A. (2009). Molecular polymorphism analysis of BMPR-IB, IGFBP-3 and POULF1 genes in Nilagiri and Mecheri sheep. Ph D. Thesis. Tamil Nadu Veterinary and Animal Sciences Univ., Chennai, India.

Talebi R., Ahmadi A., Afraz F., Sarry J., Woloszyn F. and Fabre S. (2018). Detection of single nucleotide polymorphisms at prolificacy major genes in the Mehraban sheep and association with litter size. Ann. Anim. Sci. 18(5), 685-698.

Tavakolian J. (2000). An introduction to Genetic Resources of Native Farm Animals in Iran. Animal Science Genetic Research Institute Press, Tehran, Iran.

Vacca G.M., Dhaouadi A., Rekik M., Carcangiu V., Pazzola M. and Dettori M.L. (2010). Prolificacy genotypes at BMPR 1B, BMP15 and GDF9 genes in North African sheep breeds. Small Rumin. Res. 88(1), 67-71.

Vage D.I., Husdal M., Matthew P.K., Klemetsdal G. and Boman I.A. (2013). A missense mutation in growth differentiation factor 9 (GDF9) is strongly associated with litter size in sheep. BMC Genet. 14, 1-10.

Valizadeh R. (2010). Iranian sheep and goat industry at a glance. Pp. 1-6 in Proc. Stress Manag. Small Rumin. Prod. Product Proc. Jaipur, India.

Vera J., Bravo S., Paz E. and Sepulveda Y.N. (2015). Detection of polymorphisms major gene GDF9 in corriedale flocks in Aysen region, Chile. Chilean J. Agric. Anim. Sci., ex Agro-Cien. 31(1), 15-20.

Vivekanand J.P. (2010). Screening of local sheep breeds of gujarat for presence of fecundity gene mutations by PCR-RFLP. Ph D. Thesis. Anand Agricultural Univ., Gujarat, India.

Wang W., La Y., Zhou X., Zhang X., Li F. and Liu B. (2018). The genetic polymorphisms of TGFβ superfamily genes are associated with litter size in a Chinese indigenous sheep breed (Hu sheep). Anim. Reprod. Sci. 189, 19-29.

Wang W., Liu S., Li F., Pan X., Li C., Zhang X., Ma Y., La Y., Xi R. and Li T. (2015). Polymorphisms of the ovine BMPR-IB, BMP-15 and FSHR and their associations with litter size in two Chinese indigenous sheep breeds. Int. J. Mol. Sci. 16(5), 11385-11397.

Wilson T., Wu X.Y., Juengel J.L., Ross I.K., Lumsden J.M., Lord, E.A., Dodds K.G., Walling G.A., McEwan J.C., O’Connell A.R., McNatty K.P. and Montgomery G.W. (2001). Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biol. Reprod. 64(4), 1225-1235.

Zamani P., Abdoli R., Deljou A. and Rezvan H. (2015). Polymorphism and bioinformatics analysis of growth differentiation factor 9 gene in Lori sheep. Ann. Anim. Sci. 15(2), 337-348.

Zhang C.S., Geng L.Y., Du L.X., Liu Z.Z., Fu Z.X., Feng M.S. and Gong Y.F. (2011). Polymorphic Study of FecX^G, FecG^H and FecB mutations in four domestic sheep breeds in the lower yellow river valley of China. J. Anim. Vet. Adv. 10(17), 2198-2201.

Zuo B.Y., Qian H.G., Liu J.S., Li Y.H., Wuyun B., Aiken J.,Ying S.J., Wang Z.Y., Noor N., Wang X., Guo Z.Q. and Wang F. (2012). Detection of the 10 mutations of BMPRIB, BMP15 and GDF9 gene in German Mutton Merino sheep. J. Nanjing Agric. Univ. 35(3), 114-120.

Zuo B.Y., Qian H.G., Wang Z.Y., Wang X., Noor N., Bayier A., Ying S.J., Hu X.L., Gong C.G., Guo Z.Q. and Wang F. (2013). A study on BMPR-IB genes of Bayanbulak sheep. Asian-Australasian J. Anim. Sci. 26(1), 36-42.