چندشکلی مرتبط با برخی از صفات گوشت در نژادهای گوسفند مصری

Subject Areas : Camel

کا.ف. ماهروس ¹ , م.اس. حسنان ² , م. ابدل مردی ³ , اچ.آی. شافی ⁴ , اچ.ای. روشدی ⁵

1 - Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
2 - Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
3 - Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt
4 - Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
5 - Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt

Received: 2014-05-25 Accepted : 2014-11-15 Published : 2015-09-01

Keywords: PCR-RFLP, گوسفند, چندشکلی ژنتیکی,

Abstract :

چندشکلی ژنتیکی برخی از ژن­های مرتبط با تولید گوشت در سه نژاد گوسفند مصری (بارکی، رحمانی و اُسیمی) مورد مطالعه قرار گرفت. ژن­های کاندیدا شامل کالپاستاتین، میوستاتین، دی­آسیل گلیسرول استیل ترانسفراز 1، پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی و ژن باروری برولا بودند. برای این هدف از روش PCR-RFLP استفاده شد. چند‌شکلی در ژن­های کالپاستاتین، میوستاتین و دی­آسیل گلیسرول استیل ترانسفراز 1 مشاهده گردید ولی در ژن­های پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی و ژن باروری برولا چند‌شکلی دیده نشد. کالپاستاتین با کمک آنزیم MspI هضم شده و دو ژنوتیپ MM و MN مشاهده شد. بیشترین فراوانی آللی مربوط به آلل M بود. جایگاه کالپاستاتین با کمک آنزیم NcoI نیز هضم شده و ژنوتیپ­های MM و MN دیده شدند. ژنوتیپ NN در هر دو آنزیم MspI و NcoI در کلیه نژادها دیده نشد. میوستاتین توسط DraI هضم شده و دو ژنوتیپ AB و BB مشاهده گردید. ژنوتیپ AA تشخیص داده نشد. بیشترین فراوانی آللی مربوط به آلل B بود. دی­آسیل گلیسرول استیل ترانسفراز 1 توسط AluI هضم شد و دو ژنوتیپ CC و CT ایجاد نمود. بیشترین فراوانی آللی مربوط به آلل C بود. ژنوتیپ CT می­تواند بیانگر وجود چربی بین ماهیچه­ای و ماربلینگ ماهیچه­ای متوسط در نژادهای گوسفند مصری باشد. هر کدام از دو جایگاه ژنی دیگر (پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی و ژن باروری برولا) تنها یک ژنوتیپ (ژنوتیپ BB در پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی هضم شده با آنزیم HaeIII و ژنوتیپ ++ در ژن باروری برولا هضم شده با آنزیم AvaII) داشتند. بنابراین استفاده از این جایگاه­ها در برنامه­های انتخاب توصیه نمی­شود. نتایج آنالیز مربع کای نیز نشان داد که این سه نژاد گوسفند مصری در تعادل هاردی-واینبرگ بودند.

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