تنوع ژنتیکی ژن سیتوکروم b در سه نژاد بز بومی در جاوه مرکزی اندونزی

Subject Areas : Camel

س. سوتوپو ¹ , ا. پوربوواتی ² , ا. کورنیانتو ³

1 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
2 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
3 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia

Received: 2017-06-10 Accepted : 2017-08-31 Published : 2018-12-01

Keywords: تنوع ژنتیکی, بز بومی, DNA‌ میتوکندریایی,

Abstract :

این پژوهش برای توضیح تنوع ژنتیکی و ارتباط فیلوژنیکی سه نژاد بز بومی در جاوه مرکزی اندونزی بر اساس توالی ژن سیتوکروم b (cyt b) انجام شد. نمونه‌های خون به ترتیب از 19، 15 و 12 رأس از نژادهای Kacang، Kejobong وEttawa Grade، به عنوان مواد پژوهش استفاده شدند. برهم‌گذاری چندگانه ژن cyt b توسط مقایسه نژادهای بز تحت مطالعه و یک توالی ارائه شده در بانک ژن (کاپرا هیرکوس، شماره دسترسی AB004072) انجام شد. از 16 جایگاه متفاوت جفت بازی یافت شده، 12 جایگاه وجود دارد که می‌تواند به عنوان نشانگر برای تشخیص سه نژاد بز بومی در اندونزی با کاپرا هیرکوس استفاده شوند. فاصله ژنتیکی درمیان بزهای بومی در مقایسه با فاصله ژنتیکی بین بزهای بومی با توالی ژن بانک کاپرا هیرکوس کاملاً نزدیک بود. درخت فیلوژنی دو خوشه اصلی را نشان داد، که خوشه A برای کاپرا هیرکوس و خوشه B برای سه نژاد بز بومی اندونزی بود.

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