ارزیابی شیمیایی و هیستوپاتولوژیکی در کبد جنین جوجه‌های تیمار شده با یک فرمولاسیون دلتامترین تجاری
Subject Areas : Camel
1 - Department of Zoology, IIS University, Jaipur, Rajasthan, India
2 - Department of Zoology, IIS University, Jaipur, Rajasthan, India
Keywords: دلتامترین, بیوشیمیایی, جنین جوجه‌ها, هپاتوتوکسیک, پاتولوژیکی, ساخت توده تیروئید,
Abstract :
در این مطالعه اثرات هپاتوتوکسیک دلتامترین با فرمولاسیون Decis® بر تکامل جنین Gallus domesticus بررسی شده است. تخمهای منجمد شده به مدت 60 دقیقه در دمای 37 درجه سلسیوس در سه غلظت مختلف دلتامترین (5/12، 25 و 50 میلیگرم در لیتر) در زمانهای پیش از انکوباسیون (0=ED) و روز چهارم انکوباسیون (4=ED) غوطهور شده و تا روز جنینی (16=ED) انکوباسیون گردیدند. تیمار 50 میلیگرم دلتامترین در 0 =ED و 4 =ED به ترتیب کاهش معنی داری در محتوای پروتئین کل و گلیکوژن کل کبد نشان دادند. ولی محتوای گلوتاتیون در هر دو ED در همه غلظتها کاهش یافت. در بین آنزیمها، فعالیت آلکالین فسفاتاز کبدی به طور معنی داری در 25 و 50 میلیگرم در لیتر دلتامترین در زمانهای 0 =ED و 4 =ED به طور معنی داری افزایش یافته ولی فعالیت گلوتامات پیرووات ترانس آمیناز تنها در غلظت 50 میلیگرم در لیتر دلتامترین و و 4 =ED افزایش معنی داری نشان داد. در ارزیابی هیستوپاتولوژیکی، ضایعات سلولی متوسط تا زیاد در بخش‌هایی از کبد جنینهای تیمار شده با حشرهکش دیده شد. این نتایج نشان داد با افزایش بیشتر غلظت دلتامترین مورد استفاده، میزان اغلب این تغییرات نیز شدیدتر بود. تغییرات پاتولوژیکی مشاهده شده شامل دژنره شدن و نکروز سلولهای کبدی، واکوئله شدن سیتوپلاسمی، توسعه و پرخونی فضاهای سینوزوئید عدم فیلتراسیون لوکوسیتها و گشادی و پرخونی سیاهرگ میانی بودند.
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