خصوصیات اسپرم منی منجمد گاو میش‌های نر و باروری در شرایط مزرعه
Subject Areas : Camel
کا.جی.ام. محمود
1
(Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt)
آ.آ.ای. ال-سکاری
2
(General Organization for Veterinary Services, Dokki,Giza, Egypt)
ام.ای.آ. آبو ال-روس
3
(Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt)
آ.دی. عبد الغفار
4
(Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt)
ام. ناویتو
5
(Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt)
Keywords: گاو میش نر, لقاح داخل آزمایشگاهی, یکپارچگی غشاء, کیفیت اسپرم,
Abstract :
کیفیت منی و ارتباط آن با باروری از اهمیت زیادی برخوردار است. هدف این مطالعه ارزیابی ارتباط بین خصوصیات اسپرم منجمد یخ‌گشایی شده و باروری از طریق تلقیح مصنوعی بود. نمونه‌های منی از چهار گاو میش تهیه شدند. حجم منی و غلظت اسپرم به طور معنی‌داری میان نرها متفاوت بود، در حالی که قابلیت تحرک‌پذیری اسپرم و درصد زنده‌مانی آن تفاوتی با اسپرم‌های تازه نداشت. اسپرم‌های منجمد یخ‌گشایی شده برای تحرک‌پذیری، زنده‌مانی، غیر طبیعی بودن، یکپارچگی غشاء و باروری روی دام مورد ارزیابی قرار گرفتند. تفاوت معنی‌داری در پارامترهای اسپرم گاومیش‌های نر بعد از یخ‌گشایی دیده شد. تفاوت‌های خیلی معنی‌داری در یکپارچگی غشاء بین اسپرم تازه در مقایسه با اسپرم منجمد تشخیص داده شد. نرخ آبستنی به طور معنی‌داری بین گاومیش‌های نر تفاوت داشت. همبستگی معنی‌داری بین تحرک‌پذیری و غیر طبیعی بودن اسپرم (64/0r=-) و یکپارچگی غشاء اسپرم (64/0r=-) یافت شد. همبستگی منفی (73/0r=-) معنی‌داری بین غیر طبیعی بودن اسپرم و یکپارچگی غشا گزارش شده است. علاوه بر این، یک همبستگی بین نرخ آبستنی و درصد اسپرم‌های زنده (65/0r=-) نیز گزارش شده است. در این مطالعه، تحرک‌پذیری اسپرم با غیر طبیعی بودن و یکپارچگی غشاء ارتباط داشت. درصد اسپرم زنده، تنها پارامتر همبسته با باروری بود. تحرک‌پذیری و درصد اسپرم زنده می تواند به عنوان یک معیار پیش‌بینی در ارزیابی اسپرم استفاده شود.
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