استفاده از چند‌شکلی‌های ریزماهواره‌ای موجود در ژن Ovar-DRB1 جهت تعیین مقاومت ژنتیکی نسبت به نماتودهای دستگاه گوارش در گوسفندان دنبه‌دار قزل

Subject Areas : Camel

ر. حاجی علیزاده ولیلو ¹ (Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
س.ع. رافت ² (Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
م. فیروزآمندی ³ (Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran)
م. ابراهیمی ⁴ (Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)

Received: 2015-12-23 Accepted : 2016-02-28 Published : 2016-12-01

Keywords: نماتودهای دستگاه گوارش, گوسفند قزل, چندشکلی ریز ماهواره‌ای, ژن <i>Ovar-DRB1</i>,

Abstract :

این پژوهش به منظور شناسایی دام­های دارای مقاومت ژنتیکی نسبت به نماتود‌های دستگاه گوارش (GIN) با استفاده از چندشکلی­های ریزماهواره­ای موجود در ژن Ovar-DRB1 بره‌های نژاد قزل ایرانی طراحی شد. در پژوهش حاضر 120 بره نر قزل 4 تا 6 ماهه به صورت تصادفی از 6 گله گوسفند متفاوت در استان آذربایجان شرقی انتخاب شدند (تعداد 20 گوسفند در هر گله). این بره‌ها به صورت طبیعی با GIN‌ها آلوده شده و نمونه‌های مدفوعی هر حیوان نیز جهت شمارش تعداد تخم­انگل موجود در مدفوع (FEC) به صورت دوبار در هفته و با یک هفته فاصله جمع‌آوری شد. نمونه‌های خونی نیز جهت استخراج DNA جمع‌آوری شدند و PCR به منظور افزوده­سازی اگزون شماره ­2 و توالی­های ریز‌ماهواره‌ای موجود در اینترون شماره­2 ژن Ovar-DRB1 انجام شد. داده‌ها با استفاده از رویه مختلط در نرم افزار SAS آنالیز شدند. در پژوهش حاضر 24 ژنوتیپ و 20 آلل برای ژن Ovar-DRB1 شناسایی شدند. نتایج نشان دادند که حضور آللی به طول 510 جفت ­باز (که آلل F نامیده شد) در هر دو دام‌های هموزیگوت و هتروزیگوت دارای رابطه­ قابل­توجه معنی­داری (01/­0P<) با کاهش FEC دارد، در حالیکه حضور آللی به طول 506 جفت­باز (که آلل E نامیده شد) در دام­های هموزیگوت نیز دارای رابطه معنی­داری (01/­0P<) با افزایش میزان  FECمی باشد. بنابراین این پژوهش رابطه قابل­توجه معنی­داری را بین چندشکلی­های ریز‌ماهواره‌ای موجود در ژن Ovar-DRB1 و مقاومت به GIN در بره‌های نژاد قزل نشان داد.

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