Somatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)

Subject Areas : Journal of Ornamental Plants

B. Kaviani ¹ (Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran)

Received: 2015-08-10 Accepted : 2015-08-10 Published : 2015-12-01

Keywords: Medicinal Plants, In vitro culture, plant growth regulators, 2, Micropropagation, 4-D,

Abstract :

In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0 and 0.5 mM IBA for cotyledons. Embryos were observed in embryonic axes explants cultured on MS medium containing 1mM 2, 4-D. No somatic embryos were seen on cotyledons explants.