تاثیر محلولپاشی سیلیکون بر واکنش‌های فیزیولوژیکی داوودی (Dendranthema ×grandiflorum) در دو مرحله رشد مختلف
Subject Areas : Journal of Ornamental Plantsهادی حاجی زاده 1 , زهره جبارزاده 2
1 - Department of Horticultural Science, College of Agriculture, Urmia University, P.O. Box 165, Iran
2 - Department of Horticultural Science, College of Agriculture, Urmia University, P.O. Box 165, Iran
Keywords: سیلیکات سدیم, آسکوربات پراکسیداز, کاتالاز, پراکسید هیدروژن, داوودی, سیلیکات کلسیم, گایاکول پراکسیداز,
Abstract :
این پژوهش برای ارزیابی واکنش های فیزیولوژیکی داوودی به محلولپاشی سیلیکون در شرایط بدون تنش انجام شد. این پژوهش به صورت دو آزمایش جداگانه (در مرحله 5-4 برگی و 12-8 برگی) به صورت فاکتوریل در قالب طرح کاملا تصادفی با دو فاکتور نوع سیلیکون (در دو نوع: سیلیکات سدیم و سیلیکات کلسیم) و غلظت سیلیکون (در 5 غلظت صفر، 50، 100، 150 و 200 میلی گرم در لیتر) در محیط کشت کوکوپیت- پرلیت (به نسبت حجمی 1:1) با 4 تکرار و 2 مشاهده انجام شد. ویژگی های فیزیولوژیکی نظیر فعالیت آنزیم های کاتالاز، آسکوربات پراکسیداز، گایاکول پراکسیداز، میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن اندازه گیری شدند. نتایج این پژوهش نشان داد که محلولپاشی با سیلیکات سدیم و کلسیم، میزان مالون دی آلدئید و فعالیت آنزیم های کاتالاز، آسکوربات پراکسیداز و گایاکول پراکسیداز را افزایش داد ولی پرولین و پراکسید هیدوژن کاهش یافتند. در کل، در مرحله 12-8 برگی، غلظت های زیاد سیلیکات سدیم (150 و 200 میلی گرم در لیتر) در افزایش فعالیت آنزیم های آنتی اکسیدانی و کاهش میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن موثرتر بود. در مرحله 5-4 برگی، سیلیکات سدیم با غلظت 100 میلی گرم در لیتر، فعالیت آنزیم‌های آنتی اکسیدانی را افزایش داد و سیلیکات کلسیم با غلظت 150 میلی گرم در لیتر، میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن را کاهش داد.
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