Direct Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Solanum Nigrum L. A Medicinal Herb

Subject Areas : Journal of Ornamental Plants

M.S. Kavitha ¹ , E.G. Wesely ² , P. Mehalingam ³

1 - Centre for Biotechnology, Muthayammal College of Arts & Science, Rasipuram-637408, Tamil Nadu, India
2 - PG Department of Botany, A.A. Government Arts College, Namakkal, Tamil Nadu, India
3 - Research Centre in Botany, V.H.N. Senthikumara Nadar College, Virudhunagar, Tamil Nadu India

Received: 2015-08-15 Accepted : 2015-08-15 Published : 2015-03-01

Keywords: Micropropagation, BAP, Black Nightshade, NAA, Solanaceae,

Abstract :

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple shoots was obtained on MS medium supplemented with BAP (1.0 mg/l) from shoot tip (20.4±0.22) and MS medium supplemented with BAP (3.0 mg/l) from nodal explants (8.4±0.22). The in vitro regenerated shoots were rooted (8.4±0.16 roots per shoot) on MS medium supplemented with NAA (1.0 mg/l) within 2-3 weeks of culture and the regenerated plantlets could be successfully established in soil where they grow normally.