Matthiola incana Micropropagation Using Shoot Tips and Callus Induction Derived from Lamina Explants and Rooting Capacity from Callus

Subject Areas : Journal of Ornamental Plants

A. Ahmadi Hesar ¹ , B. Kaviani ² , D. Hashemabadi ³ , A.R. Tarang ⁴ , S. Bohlooli Zanjani ⁵ , M. H. Ansari ⁶

1 - M.Sc. Student, Rasht Branch, Islamic Azad University, Rasht, Iran
2 - Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
3 - Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
4 - North Biotechnology Institute, Rasht, Guilan, Iran
5 - North Biotechnology Institute, Rasht, Guilan, Iran
6 - Department of Agronomy, Rasht Branch, Islamic Azad University, Rasht, Iran

Received: 2015-08-16 Accepted : 2015-08-16 Published : 2015-09-01

Keywords: ornamental plants, Brassicaceae, organogenesis, phytohormones,

Abstract :

Tissue culture is an attractive alternative for plant propagation. Micropropagation is a technique to ensure a constant and uniform source of ornamental plants. Matthiola incana is an important ornamental species mainly cultivate by seed. Matthiola incana seeds were germinated on solid MS medium without plant growth regulators. Shoot proliferation and root formation are possible using kinetin (Kn) and naphthalene acetic acid (NAA). Shoot tips and leaf micro-cuttings derived from in vitro germinated seedlings were subcultured on solid MS medium containing Kn (0, 0.5, 1 and 2 mg l-1) and NAA (0, 0.5, 1 and 2 mg l-1) for shoot tips explants and Kn (0, 0.5 and 1 mg l-1) and NAA (0, 0.5 and 1 mg l-1) for leaf explants. Shoot tips media supplemented with 2 mg l-1 Kn without NAA and 2 mg l-1 NAA without Kn resulted in the best shoot length (1.20 cm) and root number (1.90), respectively. The callus was induced from most leaf media after four weeks of culture. MS mediums containing 0.5 mg l-1 Kn and 0.5 mg l-1. The largest number (1.94) and the highest length (16.60 mm) of roots were obtained in MS medium supplemented with 1 mg l-1 Kn+0.5 mg l-1 NAA. NAA prevented root formation originated from callus with concentration of 1 mg l-1+0.5 and 1 mg l-1 Kn.