Comparison of Rosemary (Rosmarinus officinalis L.) hydroalcoholic extract on the viability of head and neck cancer cells Line HN5 and neuronal progenitor cells of Mouse

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Tayebeh Mohammadi ¹ (Biology, Sciences, Shahid Chamran of Ahvaz)
Elham Hoveizi ² (Biology, Sciences, Shahid Chamran of Ahvaz)

Received: 2017-09-27 Accepted : 2017-12-30 Published : 2017-08-23

Keywords: cancer, Rosemary, cell viability, PNCs,

Abstract :

Cancer is a main health problem worldwide and the number of cancer patients is increasing annually. Cancer treatment needs new anticancer medicines because of drug resistance. Rosemary is one of the herbal medicines which its anti-cancer effects have been reported. The purpose of the present study was to evaluate rosemary extract effect on HN5 cancer cells viability in comparison with neuronal progenitor cells(NPCs). NPCs were obtained from 17 days pregnant mice by enzymatic digestion method. These cells and HN5 cells were treated with 50, 100, 200 and 500 µg/ml of rosemary extract for 24, 48 and 72 hours. Their viability was measured using MTT assay. Results showed that rosemary extract decreased HN5 cells viability in 100, 200 and 500 µg/ml concentrations which were significant in comparison with NPCs. The extract increased NPCs proliferation rate in 50 and 100 µg/ml concentration and decreased their viability in 500 µg/ml concentration. Rosemary extract can decrease cell viability as a dose and time dependent manner but this effect also depends on the cell type such as its killing effect on cancer cells was more. 

References:

[1].   Abdanipour AR, Shadman B, Salimi Nanehkaran F, Bonabi R, Norian A. 2014. In Vitro evaluation of hydroethanolic extracts of citrus aurantium on proliferative rate of NSCs. J Babol Univ Med Sci; 16 (7): 36-40.

[2].   Ahmad, H.H.; Hamza, A.H.; Hassan, A.Z.; Sayed, A.H., 2013. Promising therapeutic role of Rosmarinus officinalis successive methanolic fraction against colorectal cancer. Int. J. Pharm. Pharm. Sci; 5: 164–170.

[3].   Al-Gholam MA, Nooh HZ, El-Mehi AE, El-Barbary Ael-M, Fokar AZ. 2016. Protective effect of rosemary on acrylamide motor neurotoxicity in spinal cord of rat offspring: postnatal follow-up study.  Anat Cell Biol. ; 49 (1):34-49.

[4].   Alexandrov, K.; Rojas, M.; Rolando, C. 2006. DNA damage by benzo(a)pyrene in human cells is increased by cigarette smoke and decreased by a filter containing rosemary extract, which lowers free radicals. Cancer Res, 66, 11938–11945

[5].   Chandler F. 1995. Memory stimulate: herbal medicine. Can. Pharm. J.; 28: 40-53.

[6].   Dermarderosian A. 2001. The Review of Natural Products. First ed. Missouri: Wolters Kluwer Co.; 512-13.

[7].   Douglas E, Peterson JA, Ambrosis D. 1994. Nonsurgical management of head and neck cancers patients. Dent Clin of North Am; 38 (3): 425-445.

[8].   Ertel A, Verghese A, Byers SW, Ochs M and Tozeren A. 2006. Pathway-specific differences between tumor cell lines and normal and tumor tissue cells.Molecular Cancer.; 5:55. doi:10.1186/1476-4598-5-55.

[9].   Ganz PA. 2009. Survivorship: adult cancer survivors. Prim Care; 36(4):721-741.

[10].Ghapanchi J. Mortazavi M. Parhiz H. 2004. Analytic Evaluation of the Prevalence of Head and Neck Cancers among Patients with Different Kinds of Cancers Visited in Radiotherapy Department of Nemazee Hospital, 2003-2004. Shiraz Univ. Dent. J.; 5(1, 2): 97-105.

[11].González-Vallinas  M,  Molina S, Vicente G, Zarza V, Martín-Hernández R, et al. 2014. Expression of MicroRNA-15b and the Glycosyltransferase GCNT3 Correlates with Antitumor Efficacy of Rosemary Diterpenes in Colon and Pancreatic Cancer. PLoS ONE; 9: e98556.

[12].Hanahan D, Weinberg RA. 2011. Hallmarks of Cancer: The Next Generation. Cell.; 144: 646–674.

[13].Hosseinzadeh H, Nourbakhsh M. 2003. Effect of Rosmarinus officinalis L. aerial parts extract on morphine withdrawal syndrome in mice. Phytother. Res.; 17:938-41.

[14].Irish JC, Bernstein A. 2000. Oncogenes in head and neck. Cancer Laryngoscope; 103: 42-52.

[15].Kontogianni VG, Tomic G, Nikolic I, Nerantzaki AA, Sayyad N, et al. 2013. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity. Food Chem.; 136: 120–129.

[16].Kosaka K, Yokoi T. 2003. Carnosic acid, a component of rosemary (Rosmarinus officinalis L.), promotes synthesis of nerve growth factor in T98G human glioblastoma cells. Biol. Pharm. Bull.; 26:1620-2.

[17].Moore J, Megaly M, MacNeil  AJ, Klentrou P, Tsiani E. 2016. Rosemary extract reduces Akt/mTOR/p70S6K activation and inhibits proliferation and survival of A549 human lung cancer cells. Biomed. Pharmacother.; 83:725–732.

[18].Moore J, Yousef M, Tsiani E. 2016. Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols. Nutrients; 8:731. doi:10.3390/nu8110731.

[19].Okano H. 2010. Neural stem cells and strategies for the regeneration of the central nervous system. Proc Jpn Acad Ser B Phys Biol Sci.; 86:438-50.

[20].Okumura N, Yoshida H, Nishimura Y, Kitagishi Y, Matsuda S. 2012. Terpinolene, a component of herbal sage,downregulates AKT1 expression in K562 cells. Oncol. Lett.; 3:321–324.

[21].Ozcan M. 2003. Antioxidant activities of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil. J. Med. Food.; 6:267-270.

[22].Peng CH, Su JD, Chyau CC, Sung TY, Ho SS, Peng CC, Peng RY. 2007. Supercritical Fluid Extracts of Rosemary Leaves Exhibit Potent Anti-Inflammation and Anti-Tumor Effects. Biosci. Biotechnol. Biochem.; 71:2223–2232.

[23].Petiwala SM, Berhe S, Li G, Puthenveetil AG, Rahman O, Nonn L, Johnson JJ. 2014. Rosemary Rosmarinus officinalis) Extract Modulates CHOP/GADD153 to Promote Androgen Receptor Degradationand Decreases Xenograft Tumor Growth. PLoS ONE; 9: e89772.

[24].Shabtay  A, Sharabani H, Barvish Z, Kafka M, Amichay D, Levy J et al. 2008. Synergistic Antileukemic Activity of Carnosic Acid-Rich Rosemary Extract and the 19-nor Gemini Vitamin D Analogue in a Mouse Model of Systemic Acute Myeloid Leukemia. Oncology;75:203–214

[25].Slamenova D, Kuboskova  K, Horvathova E, Robichova S. 2002. Rosemary-stimulated reduction of DNA strand breaks and FPG-sensitive sites in mammalian cells treated with H2O2 or visible light-excited Methylene Blue. Cancer Lett.; 177:145–153.

[26].Sotelo-Felix JI, Martinez-Fong D, Muriel P, Santillan RL, Castillo D, Yahuaca P. 2002. Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride- induced acute hepatotoxicity in the rat. J. Ethnopharmacol.; 81:145-154.

[27].Tamm C, Duckworth J, Hermanson O, Ceccatelli S. 2006. High susceptibility of neural stem cells to methylmercury toxicity: effects on cell survival and neuronal differentiation. J Neurochem; 97:69-78.

[28].Wood NK, Goaz PW. 1997. Differential diagnosis of oral and maxillofacial lesions. 5th ed., Mosby Com.; 587-593.

[29].Yan M, Li G, Petiwala SM, Householter E,Johnson JJ. 2015. Standardized rosemary (Rosmarinus officinalis) extract induces Nrf2/sestrin-2 pathway in colon cancer cells. J. Funct. Foods; 13: 137–147.

[30].Zargari A. 1990. Herbal medicines. Tehran University publication center., 4^th edition, 71-76 (In Persian).