Evaluation of the level of FGF12 gene expression in tissue samples of patients with colorectal cancer

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Golshan Khalafian ¹ , Malihe Entezari ²

1 - Department of Genetics,Faculty of advanced sciences and technology,Tehran medical sciences,Tehran,Iran
2 - Department of Genetics,Faculty of advanced sciences and technology,Tehran medical sciences,Tehran,Iran

Received: 2019-02-24 Accepted : 2019-04-15 Published : 2019-08-23

Keywords: biomarker, FGF12, colorectal cance,

Abstract :

Background: Colorectal cancer (CRC) is the fourth-leading cause of cancer-related deaths Many studies have indicated that the signaling pathway of fibroblast growth factor is one of the effective pathways for growth and metastasis of tumor cells. Hence, the objective of this study was to evaluate the level of FGF12 gene expression in tumor tissue and adjunct tumor tissue in patients with colorectal cancer. Methodology: Total RNA was extracted from 30 tumor tissues and 30 adjunct normal tissues in patients with colorectal cancer based on Qiagen RNeasy mini kit protocol and the cDNA was synthesized based on RevertAid First Strand cDNA Synthesis kit protocol. Specific primers for FGF12 and β-actin (internal control) genes were designed. Finally, the mRNA level of the studied genes was measured by using quantitative real-time PCR method. Data were analyzed using REST2009 and SPSSv23 software. Results: Comparing the mean expression of FGF12 gene in the tumor tissue compared to that in the adjacent normal tissue showed that a significant relationship was seen between two groups in terms of FGF12 gene expression with a total expression of 3.98 However, the expression of FGF12 gene was not significantly correlated with other clinical factors (P> 0.05). Conclusion: over-expression of FGF12 gene in colorectal cancer in normal tissue and increase in expression of FGF12 gene in this group were more in high-stage group compared to those in low-stage group, indicating that FGF12 gene may be used as a diagnostic biomarker for colorectal cancer.