Phytochemical stability of Satureja bachtiarica after cryopreservation by Desiccation

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

leila Ghaffarzadeh namazi ¹ (iran ardabil university of mohaghegh ardabili)

Received: 2021-11-04 Accepted : 2022-05-01 Published : 2022-05-22

Keywords: Essential oil, Keyword: Cryopreservation, S. bachtiarica,

Abstract :

AbstractIn recent years, our country has drawn an increasing amount of attention to medicinal plants. Because different plant species Savory inappropriate harvesting of natural areas by humans, and the growing threat of biotic and abiotic stresses following the work resulted in reduced genetic diversity of a species is, a species of genetically keep reserves Curb upper's. Seed storage techniques at very low temperature (-196 °C) using liquid nitrogen is one way of maintaining germplasm with much less cost and without loss of viability on storage and it is useful when it does not lead to changes in the chemical composition of essential oil in the desired plant. In order to investigate the phytochemical stability and compare the type and amount of identified compositions, the treated seeds were transferred to the liquid nitrogen at –196°C for 1 week as compared to the control. The aerial tissues of in vitro-grown plants were collected and dried in the laboratory. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Twelve compounds were recognized in the essential oil of mentioned plant in the cryopreservation. Results indicated that regarding the number, type and percentage of compounds existing in the essential oils, there was no difference between the control and cryopreservation treatment so by using the cryopreservation technique, the seeds of this valuable endangered species can be preserved for a long period.