Investigation of hydroalcoholic extract effect of Cordia Myxa fruit on the expression of BCL-2 and BAX genes in the apoptotic pathway and cell proliferation in human lung cancer cell line.
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
NAHID ASKARI
1
,
Kian Aghaabbasi
2
,
elham rezvannejad
3
1 - Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
2 - Department of Biotechnology,University of Guilan, University Campus 2 ,Rashat, Iran
3 - Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
Keywords: "Cordia myxa L", " Lung Cancer cell line", " BAX gene", "BCL-2 gen",
Abstract :
Cancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on cellular proliferation and apoptosis gene expression (BCL-2 and BAX) in lung cancer cell line (A549). The plant was collected and Hydroalcoholic extract was prepared by macerating method. Various concentrations of Cordia myxa extracts (of 0.25, 0.5, 1, 2, 4 mg/mL) obtained to treat lung cancer and fibroblast cell lines. Cells were examined by MTT-assay and Real Time RT-qPCR to determine the plant extract-induced cytotoxicity in cell lines with and without treatment The results showed that, the concentration of ≥2 mg/ml extract had a significant lethal effect on cancer cells. The expression of BAX gene in the treated cell line increased and the expression of BCL-2 gene decreased compared to the control. The extract of Cordia myxa fruit didn’t show toxicity effects when used in low concentrations. However, this extract demonstrated the lower cytotoxicity against normal fibroblast cells. The combination therapy can be less toxic if both (plant extract and chemotherapeutic agent) use to treat cancers. It can be useful with fewer side effects of chemotherapeutic agents.
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