Chemical ionization of valine amino acid at atmospheric pressure using ion mobility spectrometer equipped with corona discharge ionization source

Subject Areas :

Manijeh Tozihi ¹ (Chemistry department, Science faculty, University of Zanjan, Zanjan, Iran)
Nasim Najaflu ² (Chemistry department. Science faculty. University of Zanjan, Zanjan. Iran)
Hamed Bahrami ³ (aDepartment of Chemistry, University of Zanjan, Zanjan 45371-38791, Iran)

Received: 2023-05-29 Accepted : 2023-08-26 Published : 2024-03-17

Keywords: Valine amino acid, Ion mobility spectrometry, Protonation, Fragmentation, Adduct ions.,

Abstract :

In this research, chemical ionization of valine amino acid in atmospheric pressure has been investigated through its interaction with hydronium and ammonium ions by using ion mobility spectrometer equipped with corona discharge ionization source. It was found that in the presence of hydronium as reactant ion and ammonium as dopant, the ionization of valine took place through its protonation, fragmentation of protonated molecule, and also formation of adduct ions. At least six ionic species were identified as product. In ion mobility spectrum of valine, two signals were assigned to the protonated valine and its symmetric proton-bound dimer, respectively. Identification of two other signals were conducted by comparison of the ion mobility spectrum of valine and isobutylamine. Using mass-mobility correlation equation based on two standard masses, two other signals were assigned. One of them was attributed to an ionic cluster obtained from the interaction of the ionic fragment with the neutral molecule of valine and the other one was related to a carbocation resulting from the fragmentation of protonated valine through the elimination of water. By the study of time, evolution of product and reactant ions signals and also the change of cell temperature, the proposed origin of the signals was confirmed. Therefore, the identification of ion products without need to couple the ion mobility spectrometer to the mass spectrometer was performed.

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