Investigating the effects of some growth regulators in vitro culture (Rhododendron yakusimanum L.)

Subject Areas : Plant Biology

Mohammad Ebrahimzadeh ¹

1 - Department of Biology, Varamin-Pishva Branch, Islamic Azad university, Varamin, Iran

Received: 2024-02-13 Accepted : 2024-08-11 Published : 2025-02-08

Keywords: In vitro culture, Rhododendron yakozimanum, growth regulators, organogenesis,

Abstract :

Rhododendron yakusimanum (Rhododendron yakusimanum L.) is one of the ornamental plants in the world that is propagated by the traditional method of cutting leafy stems. Unfortunately, this method is not completely successful and cutting losses are high. Also, in this method, it is possible to spread infected plants. The surface contamination of the samples was removed using 70% alcohol and 2% sodium hypochlorite for 20 seconds and 15 minutes, respectively. For the purpose of callogenesis, the sterilized pieces were cultured in the medium Anderson (1978-1980) containing different concentrations of growth regulators 2,4-D and 2ip, and the largest callus volume was at the concentration of 2ip (5) mg/lit and 2,4-D (0.5 mg/lit) in lighting. For the purpose of organogenesis, the resulting callus were transferred to environments designed with IAA and 2ip hormones in different concentrations, and the best environment for aerial organogenesis was observed at the concentration of 2ip (5 mg/lit and 0.5mg/lit) IAA. became The rooting of the elongated branches was investigated in different environments, and the rooting took place only in the environment outside the glass in the perlite medium.

References:

1. Almeida R, Gonçalves S, Romano, A. 2005.In vitro micropropagation of endangered Rhododendron ponticum L. ... Iapichino G, McCulloch S, Chen THH (1992)
2. Anderson, W.C. 1984. A revised tissue culture for shoot multiplication of Rhododendron. Journal of the American Society for Horticultural science. 109:3, 343347, 8 ref.
3. Bhojwan, S. S; Razdan, M. K. 1992. Plant tissue culture; theory and practice, Elsevier science publisher B. V. or right reserved.
4. Blazich, F. A., C.G. Giles, and C.M. Hammerle. 1986. Micropropagation of Rhododendron chapmanii. Journal Environment Horticulture. 4: 2629.
5. Blessington, IM.; Garvery, E. J.; Howell, L. M. 1981, Effects of application methods of controlled release fertilizers on growth and quality of Rhododendron obutsum Hinodegiri grown in various media. Horscience, 16: 5, 676–677.
6. Bojarczuk, k. 1989, micropropagation of Rhododendron. Axta Hortculture. 241-247.9 ref.
7. Briggs. B. A., 1988. Micropropagation of azalea using thidiazuron. Acta Horticulture 227: 330333.
8. Briggs, B. A.; Mc culloch, s. M.; caton, L. A; Septhmann, W. 1994. In votro propagation of Rhododendron. Acta Horticutturae. 364: 2126.
9. Chee, R. 1985. The history of Rhododendron micropropagarion provides some tipsfor success. American Nurseryman 5: 4247.
10. Douglas G. C. 1984. Propagation of eight cultivars of Rhododendrron in virto using agar-solidified and liqid media and direct rooting of shoot in vivo. Scientia Horticulture, 24:337347.
11. Economou, As: Read, PE., Spanoudaki, Mj. 1988. Azalea regeneration from callus cullure. Acta-horticulture. 226 vol. I, 209216, 6 ref.
12. Eeckhaut, T. Van Huylenbroeck, J. De Scheepper, S. Van Labeke, M. 2006. Breeding for polyploidy Belgian azalea (Rhododendron simsii hybrids). Acta Hort. 714:113-118
13. Pierike, R. L. M. 1987. In vitro culture of higher plant. Handbook
14. Samyn, G. De Schepper, S. Van Bockstaele, E. 2002. Advantitious shoot regeneration and appearance of sports in several azale cultivars. Plant Cell Tissue Organ Cult. 70:223-227
15. Tom Eeckhaut, T. Kristien Janssens, Ellen De Keyser. Jan De Riek. 2010. Micropropagation of Rhododendron Methods. Mol Biol 589:141-152