Composition and antibacterial activity of the chemical essential oil of Foeniculum vulgare and Eucalyptus to control some important plant pathogenic bacteria

Subject Areas : Bacteriology

mitra omidi nasab ¹ (PhD student in Plant Pathology, Bu-Ali Sina University, Department of Plant Protection, Faculty of Agriculture)
Milad Aeini ² (Department of plant protection, faculty of Agriculture, Shahid Chamran university of Ahvaz, Ahvaz, Iran)

Received: 2019-07-24 Accepted : 2019-10-09 Published : 2020-02-20

Keywords: Pathogenic bacteria, Antioxidant, Antibacterial, Essential oil,

Abstract :

One of the most significant current discussions is approaching the antibacterial activity of essential oil to control plant pathogenic agents. Current study aimed to characterize chemical composition and antibacterial activity of Foeniculum vulgare and Eucalyptus to control some plant pathogenic bacteria in-vitro. In the present study, essential oil components of Foeniculum vulgare and Eucalyptus were subjected to Gas chromatography–mass spectrometry analysis, their minimum inhibitory concentration and antibacterial inhibitory on some plant pathogenic bacteria were determined. GC-MS analysis showed that the main component of the essential oil of F. vulgare were p-cymene, (30.18%), Cuminal (24.74%), 2-beta pinene (11.81%), while Eucalyptus were 1,8 cineol (47.87%), 1-alpha pinene (17.94%), p-cymene (7.91%) and Limonene (7.68%). Also, minimum inhibitory concentration was 3.79 µg/ml and 8.54 µg/ml for F. vulgare and Eucalyptus, respectively. Results of the antimicrobial investigation demonstrated that F. vulgare had the highest inhibition diameter of 4.4 centimeters in Clavibacter michiganensis. In Eucalyptus essential oil, highest inhibition zone (4.3 centimeters) were attributed to Erwinia amylovora. Results showed that by increasing the concentration, antibacterial activity increases. Our findings provide a rational basis of a novel emerging alternative to antimicrobial treatments in plant disease management programs.

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