Genotyping of Pseudomonas aeroginusa isolated from hospital infections

Subject Areas : Molecular Microbiology

Gholamreza Banisharif ¹ (MS.c., Department of Microbiology, Shahrekord branch, Islamic Azad University, Shahrekord, Iran.)
Hassan Momtaz ² (Associate Professor, Department of Microbiology, Shahrekord branch, Islamic Azad University, Shahrekord, Iran.)

Received: 2015-12-11 Accepted : 2016-02-09 Published : 2016-08-01

Keywords: Pseudomonas aeruginosa, rep-PCR, RAPD-PCR, Hospital infections, ERIC-PCR,

Abstract :

Background & Objectives: Pseudomonas aeruginosa is a common  opportunist  pathogen  in hospitals and the etiologic agent of   the majority of infections in human beings. This study aimed to genotyping of P. aeruginosa isolated from hospital infections. Materials &  Methods: This  cross-sectional study was  performed  on 18  isolates  of  P. aeruginosa  isolated  from hospitalized  patients in Shahrekord hospitals. We applied three different methods, RAPD-PCR, Rep-PCR and ERIC-PCR for genotyping of the isolates. Results: Based on RAPD-PCR method, overall 86 different bands of DNA with a range of 300 to 1000 bps were obtained from the under study isolates and among them, 74 bands were polymorphic. Analysis of P. aeruginosa isolates based on ERIC-PCR produced 98 bands with a range of 150-8000 bps. Overall 16 genomic profile with 30 to 86% and for a few strains, 100% similarities were produced based on Rep-PCR. Conclusion: Overall, all isolates showed polymorphic band patterns and no monochromic band was observed for the isolates. The presence of polymorphic band patterns in these techniques  shows high rates of polymorphism in the genome  of P. aeruginosa. Furthermore, the techniques used in this study are reliable approaches for genotyping of P. aeruginosa.

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