Molecular detection of Mycoplasma hominis from genital secretions of infertile men referred to the Kerman infertility center
Subject Areas : BacteriologySamira Vosooghi 1 , Babak Kheirkhah 2 , Touraj-Reza Mir-shekari 3 , Ashraf Karimi Nik 4 , Soudabeh Hamidavi Mohammadpour 5 , Naeime Mohseni Moghadam 6
1 - Pasargad Microbiology Research Group, Tehran, Iran
2 - Department of Microbiology, Baft Branch, Islamic Azad University, Baft, Iran
3 - Department of Pathology, Kerman University of Medical Science, Kerman, Iran.
4 - Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
5 - Department of Microbiology, Baft Branch, Islamic Azad University, Baft, Iran
6 - Department of Microbiology, Sirjan Science and Research Branch, Islamic Azad University, Sirjan, Iran
Keywords: Semen, Mycoplasma hominis, Polymerase chain reaction,
Abstract :
Background and Objectives: Mycoplasmal genital infections are often associated with devastating effects on quality of sperms, leading to male infertility. The aim of this study was to isolate Mycoplasma hominis from genital secretions of infertile men referred to the Kerman Infertility Center based on PCR method. Material and Methods: In this cross-sectional study, 58 semen samples were collected from the infertile men who were referred to the Infertility Center of Kerman from May 2012 to September 2012. Infertility condition was confirmed in all samples by performing a semen analysis. After genomic DNA extraction, DNAs were examined by PCR and the primers specific for both the genus Mycoplasma and species Mycoplasma hominis. Results: Overall, 22 samples (37.93%) were detected as Mycoplasml infection, among them 13 samples (22.41%) belonged to Mycoplasma hominis. Also, most of the Mycoplasmal infection were detected in the age group of 29 to 38 years. Conclusion: For the first time in Kerman, we could isolate Mycoplasma hominis from genital secretions of infertile men. The study showed that a relatively high percentage of asymptomatic infertile men were infected with this bacterium.
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