Identification and evaluation of antidiabetic activity of bacteria isolated from Persian Gulf sponges

alpha-amylase, alpha-glucosidase, sponge-related bacteria, secondary metabolites, anti-diabetes, Persian Gulf

Subject Areas : Microbial Biotechnology

Atefeh Ansarizadeh ¹ , Farshid Kafilzadeh ² , Saeid Tamadoni Jahromi ³ , Mohammad Kargar ⁴ , Mohsen Gozari ⁵

1 - Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
3 - Persian Gulf and Oman Sea Ecology Research center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar abbas, Iran
4 - Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
5 - Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran

Received: 2023-06-10 Accepted : 2023-10-07 Published : 2023-11-13

Keywords: alpha-amylase, alpha-glucosidase, sponge-related bacteria, secondary metabolites, anti-diabetes, Persian Gulf,

Abstract :

Background and purpose: Screening and identification of bacteria associated with sponges is an important step in the discovery of new drugs. The purpose of this research was to isolate and identify bacteria associated with sponges around Hormuz Island and to find bacteria that produce metabolites that inhibit the activity of alpha-glucosidase and alpha-amylase enzymes. Materials and methods: In this study, 25 samples of Haliclona and Niphatea sponges were collected from 6 stations. Identification was done based on phenotypic characteristics. Bacteria were cultured in broth nutrient medium and their secondary metabolites were extracted by ethyl acetate. The inhibition rate of metabolites against alpha-amylase and alpha-glucosidase was evaluated based on colorimetric methods. The toxicity of metabolites against normal umbilical cord endothelial cell line was investigated. The productive bacteria were identified by polyphasic taxonomy approach. Results: A total of 105 bacteria were isolated. Vibrio and Bacillus bacteria with 32.81% and 17.19% in Haliclona sp. and 19.51% and 34.15% in Niphatea sp. The metabolites extracted from 3 isolates inhibited amylase enzyme activity with IC50 values ranging from 0.248 to 366.8 µg/ml. Also, 4 isolates produced inhibitory metabolites against alpha-glucosidase enzyme in IC50 values from 159.4 to 670.9 µg/ml. Based on the results of polyphasic identification of capable isolates including Bacillus pumilus HH 165, Pseudomonas lurida HH 124, Streptomyces sp. HN 235, Bacillus tequilensis HN 231. Conclusion: In this study, 3 strains of bacteria producing inhibitory compounds, including alpha-ambellase and alpha-glucosidase enzymes, and without cytotoxicity were identified. The mentioned bacteria can be suitable candidates in diabetes studies.

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