Bioremediation potential of indigenous gram-positive bacteria isolated from contaminated soil with polycyclic aromatic hydrocarbons

Subject Areas : Environmental Microbiology

Somayeh Scandari ¹ , Mehran Hoodaji ² , Arezo Tahmourespour ³ , Atoosa Abdollahi ⁴

1 - دانشجوی دکتری، دانشگاه آزاد اسلامی، واحد خوراسگان، گروه خاک شناسی
2 - دانشیار، دانشگاه آزاد اسلامی، واحد خوراسگان، گروه خاک شناسی
3 - استادیار، دانشگاه آزاد اسلامی، واحد خوراسگان، گروه علوم پایه پزشکی
4 - استادیار، دانشگاه آزاد اسلامی، واحد خوراسگان، گروه شیمی

Received: 2012-09-20 Accepted : 2013-02-18 Published : 2013-04-01

Keywords: Polymerase chain reaction, Polycyclic aromatic hydrocarbons, Polluted soils, Bioremediation,

Abstract :

Background and Objectives: Polycyclic aromatic hydrocarbons (PAHs) were extensively spread in the environment and are regarded as one of the mutagenic and carcinogenic agents on living creatures. Among the vast variety of procedures for the elimination of contamination, biological removal is capable of transmuting pollutants into innocuous and nontoxic substances using less amount of energy, chemicals and time. The study was aimed at evaluating the possibility of growth of the indigenous bacteria isolated from oil-polluted soils, in the presence of PAH compounds in the laboratory, and also identifying them by using the method of PCR. Material and Methods: Specimens of the research were isolated from environmental gasoline and oil-polluted soils from the Isfahan City refinery. Initially, the native bacteria were separated from the contaminated soil with such compounds by utilizing a basic medium containing the concentration of 12.8 mg/l in 16 PAH compounds. Then, those bacteria which were able to grow and reproduce in the presence of the compounds identified through biochemical experiments and determination of genome sequence and consequently registered as new species. Results: The results obtained in the study substantiated that approximately 13.3% of the total heterotrophic bacteria possess a degradable ability of the hydrocarbons. After the evaluation of biochemical tests and gene sequencing, it was disclosed that the isolated indigenous bacteria belonged to Bacillus licheniformis ATHE9, Bacillus mojavensis ATHE13 and a particular species of Bacillus (ATHE10). Conclusion: The results of the present research verify the importance and proficiency of the native bacteria in the terms of the elimination of PAHs pollutions in contaminated areas.

References:

1. Nie M, Zhang XD, Wang JQ, Jiang LF, Yang J, Quan ZX, Cui XH, Fang CM, Li B. Rhizosphere effects on soil bacterial abundance and diversity in the Yellow River Deltaic ecosystem as influenced by petroleum contamination and soil salinization. Soil Biol Biochem. 2009; 41(12): 2535-2542.

2. Jacobsen CS. Plant protection and rhizosphere colonization of barley by seed inoculated herbicide degrading Burkholderia (Pseudomonas) in 2,4-D contaminated soil. Plant and Soil. 1997; 189(1): 139-144.

3. Kaksonen AH, Jussila MM, Lindstrom K, Suominen L. Rhizosphere effect of Galega orientalis in oil-contaminated soil. Soil Biol Biochem. 2006; 38(4): 817-827.

4. Johnsona DL, Anderson DR, McGratha SP. Soil microbial response during the phytoremediation of a PAH contaminated soil. Soil Biol Biochem. 2005; 37(12): 2334-2336.

5. Baek SO, Field RA, Goldstone ME, Kirk PW, Lester JN, Perry R. A review of atmospheric poly cyclic aromatic hydrocarbons: sources, fate and behavior. Water Air Soil Poll. 1991; 60: 279-300.

6. Niel P. Environment Chemistry. 2 nd .Chapman and Hall London SE1 8HN, UK. 1995.

7. World Health Organization. Selected non heterocyclic poly cyclic aromatic hydrocarbons environmental health criteria 202 international program on chemical safety WHO Geneva, 1998.

8. US-EPA. Code of Federal Regulation Title 40 part 60 Subparts D Da Db Dc. Environmental Protection Agency. Washington DC, 1997.

9. Su YH, Yang XY. Interactions between selected PAHs and the microbial community in rhizosphere of a paddy soil. Sci Total Environ. 2009; 407(3): 1027-1034.

10. Haritash AK, Kaushid CP. Biodegradation aspects of poly aromatic hydrocarbons (PAHs). J Hazard Mater. 2009; 169(1-3): 1-15.

11. Aitken MD, Stringfellow WT, Nagel RD, Kazunga C, Chen SH. Characteristics of Phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons. Can J Microbiol. 1998; 44(8): 743-752.

12. Andreoni V, Cavalca L, Rao MA, Nocerino G, Bernasconi S, Dell'Amico E, Colombo M, Gianfreda L. Bacterial communities and enzyme activities of PAHs polluted soils. Chemosphere. 2004; 57(5): 401-12.

13. Ma J, Xu L, Jia L. Degradation of polycyclic aromatic hydrocarbons by Pseudomonas sp. JM2 isolated from active sewage sludge of chemical plant. J Environ Sci. 2012; 24(12): 2141-2148.

14. Maiti A, Das S, Bhattacharyya N. High gelatinase activity of a newly isolated polycyclic aromatic hydrocarbon degrading bacteria Bacillus weihenstephanensis strain AN1. J Pharm Res. 2013; 6(1): 199-204.

15. Rhoades JD. Cation exchange capacity. Methods of soil analysis, part 2. American Society Agronomy, 1986.

16. Jacques RJ, Okeke C, Bento M, Peralba M, Camargo A. Improved enrichment and isolation of polycyclic aromatic hydrocarbons PAH–degrading microorganism in soil, using Anthracene as model PAH. Curr Microbiol. 2009; 58(6): 628-634.

17. Lee SH, Lee WS, Lee CH, Kim JG. Degradation of Phenanthrene and Pyrene in rhizosphere of grasses and legumes. J Hazard Mater. 2008; 153(1-2): 892-898.

18. Neuman G, Teras R, Monson L, Kivisaar M, Schauer F, Heipieper HJ. Simultaneous degradation of atrazine and phenol by Pseudomonas sp. strain ADP: effects of toxicity and adaptation. Appl Environ Microb. 2004; 70(4): 1907-1912.

19. Eskandary S, Tahmourespour A, Hoodaji M. Investigation of growth and removal of phenol by isolation of bacterium from industrial waste water in vitro.  Journal of Water Wastewater. 2011; 78: 78-85. [In Persian]

20. Holt G, Krieg R, Sneath A, Staley T, Williams T. Bergey’s manual of determinative bacteriology. 9^th edition. Williams and Wilkins publisher. 1994; 450p.

21. Mcpherson MJ, Muller S. Polymerase Chain Reaction, Bios scientific publisher. New York: Oxford. 2000; 250p.

22. Banks MK, Govindaraju RS, Schwab AP, Kulakow P. Field demonstration. In: Fiorenza S, Oubre CL, Ward CH (Eds.). Phytoremediation of Hydrocarbon-Contaminated Soil. Lewis Publishers, Boca Raton, FL, 2000; pp. 3-88.

23. United State Environmental Protection Agency (USEPA). Office of solid waste Washington DC 20460, polycyclic aromatic hydrocarbons. 2008.

24. Toledo FL, Calvo C, Rodelas B, Gonzalez-Lopez J. Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. Syst Appl Microbiol. 2006; 29(3): 244-252.

25. Thenmozhi R, Praveenkumar D, Priya E, Nagasathy A, Thajuddin N. Evaluation of aromatic and polycyclic hydrocarbon degrading abilities of selected bacterial isolates. Journal of Microbiology and Biotechnology Research. 2012; 2(3): 445-449.

26. Dean-Ross D, Moody JD, Freeman JP, Doerge DR, Cerniglia CE. Metabolism of anthracene by a Rhodococcus species. FEMS Microbiol Lett. 2001; 204(1): 205-211.

27. Eman AHM, Naeima MHY, Azza GF. Isolation and molecular identification of poly aromatic hydrocarbons-utilizing bacteria from crude petroleum oil samples. Afr J Microbiol Res. 2012; 6(49): 7479-7484.

28. Yuan SY, Chang SW, Chang BV. Biodegradation of poly cyclic aromatic hydrocarbons in sludge. Bull Environ Contam Toxicol. 2003; 71(3): 625-632.

29. Tebyanian H, Hassanshahian M, Karimi nik A. Identification of alkane hydroxylase gene in aliphathic-degrading bacteria isolated from waste oil. J Microb World. 2013; 5(3&4): 105-114.

30. Leys NM, Ryngaert A, Bastiaens L, Verstraete W, Top EM, Springael D. Occurrence and phylogenetic diversity of Sphingomonas strains in soils contaminated with polycyclic aromatic hydrocarbons. Appl Environ Microbiol. 2004; 70(4): 1944-1955.

31. Singer AC, Van der Gast CJ, Thompson IP. Perspectives and vision for strain selection in bioaugmentation. Trends Biotechnol. 2005; 23(2): 74-77.