The Inhibitory Effect of Doxycycline- Silver Nanoparticle Conjugate on Brucella melitensis

Subject Areas : Medical Microbiology

Mohsen Ajalli ¹ (Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Mojtaba Salouti ² (Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Hamed Alizadeh ³ (Young Researcher’s Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Zahra Heydari ⁴ (Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Hossein Hamzehei ⁵ (Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Aram Alizadeh ⁶ (Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran)

Received: 2010-05-15 Accepted : 2010-07-16 Published : 2010-07-01

Keywords: Silver nanoparticles, Antimicrobial effect, Doxycycline, Brucella,

Abstract :

Background and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver nanoparticles conjugate against Brucella meltensis 16M. Material and Methods: After preparing the doxycycline-silver nanoparticle conjugate, its antimicrobial activity against Brucella meltensis 16M was determined by Well Diffusion Agar method in Muller Hintone Agar media. Also, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Doxycycline-silver nanoparticle conjugate was determined by Macrodilution method in Muller Hintone Broth media. Finally, antibacterial effect of the nanoparticle was assayed in animal model. Results:  The results showed that Doxycycline-silver nanoparticle conjugate has antimicrobial activity against Brucella melitensis 16M in laboratory condition. In mouse model, the conjugate of Doxycycline-silver nanoparticle  could decrease effectively the Brucella melitensis load in liver. Conclusion:  This study demonstrated that Doxycycline-silver nanoparticle conjugate has synergistic effect on Brucella melitensis 16M and can be useful in treatment of brucellosis.

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