The catalytic activity of biological seeds and Acidithiobacillus ferrooxidans on the process of ammonium jarosite

Subject Areas : Microbial Biotechnology

Nasim Eftekhari ¹ , Mohammad Kargar ² , Farokh Rokhbakhsh Zamin ³ , Nahid Rastakhiz ⁴ , Zahra Manafi ⁵

1 - Ph.D. Candidate, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
2 - Professor, Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran,
3 - Assistant Professor, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran,
4 - Assistant Professor, Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran,
5 - Ph.D. Candidate, National Iranian Copper Industries Co., Sarcheshmeh Mine, Iran

Received: 2019-09-25 Accepted : 2019-10-25 Published : 2020-02-20

Keywords: Iron, Bioleaching, Jarosite, Acidithiobacillus ferrooxidans,

Abstract :

Background & Objectives: Ferric iron that commonly exists in the leaching solution needs to be removed before the recovery of copper bioleaching using methods such as jarosite seed. The objective of this study was to investigate the catalytic performance of biological seeds and Acidithiobacillus ferrooxidans in the process of jarosite formation via the biosynthesis process. Materials & Methods: Acidithiobacillus ferrooxidans was first grown in 9K medium. Jarosite seeds were synthesized using this bacterium. Then the effect of the biological activity of different seeds (5, 10 g/L) on jarosite formation was investigated. The type of jarosites synthesized was identified by X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FTIR), and scanning electron microscope (SEM) analysis. Meantime, the morphologies of jarosite crystals were studied. Results: The FTIR and XRD results showed that biosynthetic jarosite seeds are ammonium jarosite type. The amount of jarosite increased with increasing seed concentration and the induction time of precipitation decreased. The pH and Eh of culture medium with increasing seed decreased. Bacterial growth also decreased in the presence of jarosite seeds compared to medium without biological seeds. According to the results of SEM, The morphologies of ammonium jarosite crystals were significantly affected by the jarosite seeds. The jarosite crystals were precipitated with the presence of seeds that had a smooth, uniform, and larger surface than non- seeding jarosite. Conclusion: The results showed that the precipitation process of jarosite is more complete with biological seeds. The results of this study can improve the efficiency of the iron removal process in copper bioleaching and reduce production costs.

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