Effect of abscisic acid on seed germination, callus culture and somatic embryogenesis of rapeseed (Brassica napus L.) genotypes under salinity stress

Subject Areas : Journal of Plant Ecophysiology

Ali-Ashraf Mehrabi ¹ (گروه زراعت و اصلاح نباتات، دانشگاه ایلام)
Mansoor Omidi ² (گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران)
Badredin Tabatabaei ³ (گروه زراعت و اصلاح نباتات، دانشگاه صنعتی اصفهان)
زینب صفری ⁴ (دانشگاه ایلام)

Received: 2014-10-13 Accepted : 2014-12-14 Published : 2015-10-01

Keywords: Tissue culture, Callus Induction, Explant, plant growth regulator, hypocotyl,

Abstract :

Salinity is the one of the most important factors that limited growth and yield of crops. This study was developed to evaluate effects of abscisic acid (ABA) on seed germination, seedling growth and hypocotyl culture of rapeseed (Canola) genotypes at different levels of salinity. The research was arranged as a factorial experiment in a randomized complete block design with three replications. Genotypes at three levels, salinity at four levels and three levels of ABA were tested in this experiment. In a separate experiment hypocotyl explants were cultured in MS medium complimented with 2 mg lit-1 BAP (6-Benzylaminopurine) and 1 mg lit-1 NAA (Naphthalene acetic acid), under salinity and ABA treatments that mentioned in germination test. In separate consideration of ABA and salinity effects, both treatments were inhibited seed germination, seedling growth, callus induction and calli growth in all genotypes. The significant interactions between two factors indicated that genotypes had different responses to salinity in different concentrations of ABA. Most of germination traits that evaluated under different levels of ABA and salt stress differences were significant at 1% probability level. So that seeds had higher germination rate in 10 µmol/L ABA than 5µmol/L ABA in saline environments. In low levels of salinity (60, 120 mmol/L), callus induction and calli growth were performed more efficient using 5µmol/L ABA in comparison with no ABA treatment (at 1% probability level).