Pretreatment with hydrogen peroxide (H2O2) reduces the negative effects of salt stress on wheat

Subject Areas : Journal of Plant Ecophysiology

T. Jfarian ¹ (گروه زراعت و اصلاح نباتات، دانشگاه ایلام، ایلام، ایران)
M.J. Zare ² (گروه زراعت و اصلاح نباتات، دانشگاه ایلام، ایلام، ایران)

Received: 2014-11-29 Accepted : 2015-05-28 Published : 2016-01-01

Keywords: Catalase, Relative water content, Ascorbate peroxidase, Roots,

Abstract :

The purpose of this study was to investigate the effect of hydrogen peroxide pretreatment on morphological characteristics, physiological and biochemical ON wheat seedling under salt stress conditions. In this regards a greenhouse experimentation carried out as factorial based on randomized complete block design with three replications and three levels of salinity (0, 80 and 120 mM NaCl), and four levels of hydrogen peroxide ( 0, 25 , 50 , 80 mM). Hydrogen peroxide caused an increase of Catalase and ascorbate peroxidase in wheat seedling under salinity condition. This increase was associated with reducing the damage of free radicals in produced wheat. Under salinity stress pre-treated wheat with hydrogen peroxide had higher chlorophyll and relative water content. Also Hydrogen peroxide reduced the negative effect of salinity on water, height and root dry weight of wheat seedling. There was no significant effect of hydrogen peroxide on ionic leakage. Overall seed priming with hydrogen peroxide improves the physiological and biochemical characteristics of wheat and increase its resistance to salt stress conditions.