study of different percentage of alcalase enzyme on the fish protein hydrolysate properties from silver carp viscera

Subject Areas : Journal of Aquatic Sciences

tayebeh dahaz ¹ , mehdi zarei ² , hoda jasemian fard ³

1 - Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
2 - Young Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, Iran.
3 - Department of Seafood Processing, Kherad Institute Of Higher Education.

Received: 2022-08-02 Accepted : 2022-08-27 Published : 2022-08-23

Keywords: Fish protein hydrolysate, Different percentage of Alcalase, Amounts of mineral, Silver carp viscera,

Abstract :

The aim of this study was to investigate the characteristics of the protein hydrolyzate from silver carp (Hypophthalmichthys molitrix) viscera using different percentages of Alcalase. For this purpose, hydrolyzed proteins were produced using different percentages of Alcalase and the samples were analyzed for the amount of total protein, moisture, fat, ash, soluble protein, hydrolisis degree, nitrogen recovery, amounts of minerals and color parameters (brightness, redness and yellowness). According to the results, increased the amounts of percentage of enzyme to substrate led to increase the amounts of moisture, fat, nitrogen recovery and red index and decrease the amounts of ash, brightness and yellowness index. In general, by increasing the percentage of Alcalase to the substrate, to a specified amount, that in this study was 1.5 percent, the degree of hydrolysis increased, but then, a further increase in the amount of the enzyme led to reduce the degree of hydrolysis and had negative effects on the other experiments.