Investigation of mechanical and biological properties of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold reinforced with hydroxyapatite

Subject Areas :

نرجس کوپائی ¹ , Akbar Karkhaneh ²

1 - استاد دانشگاه/دانشگاه آزاد اسلامی واحد نجف آباد
2 - استاد دانشگاه/دانشگاه صنعتی امیر کبیر

Received: 2016-10-29 Accepted : 2018-12-18 Published : 2018-11-22

Keywords: scaffold, hydroxyapatite, polycaprolactone diacrylate, polyethylene glycol diacrylate, crosslink,

Abstract :

The purpose of this study is synthesised of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold in the presence of hydroxyapatite (HA) particles and investigated of mechanical and biological properties of scaffolds. . At the first step, PCL diol was acrylated with acryloyl chloride and confirmed using fourier transform infrared (FTIR).Then, the scaffolds were synthesized by radical crosslinking reaction of polycaprolacton acrylate and polyethylene glycol diacrylate (PEGDA) in the presence of hydroxyapatite (HA) particles and particulate technique with sodium chloride. The prepared samples were characterized using techniques such as scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), and dynamic mechanical thermal analysis (DMTA). Results show that increasing the ratio of PEGDA to polycaprolactone diacrylate (PCLDA) led to increase of tan δ peak and decrease of compressive modulus of the network, respectively. It was found that the incorporation of HA particles with the polymer matrices resulted in an increased of compressive modulus and a decrease of tan δ peak. Cytocompatability of the scaffolds were assessed by direct contact test and staining cell. Results indicated no toxicity, and cells attached and spread on the pore walls offered by the scaffols. Thus, the results indicated that the PCLDA/PEGDA/HA scaffolds have the potential of being used as promosing substrates in bone tissue engineering.

References:

 

[1]       م. فروغی، س. کرباسی، ر. ابراهیمی کهریزسنگی و ع. سعادت، "ارزیابی خواص فیزیکی داربست کامپوزیت نانو کریستال هیدروکسی آپاتیت/پلی هیدروکسی بوتیرات برای کاربرد در مهندسی بافت استخوان"، فرآیندهای نوین در مهندسی مواد، سال 6، صفحه 60-51، 1390.

 

[2]     D. Puppi, F. Chiellini, A. M. Piras & E. Chiellini, “Polymeric materials for bone and cartilage repairˮ, Progress in Polymer Science, Vol. 35, pp. 403–440, 2010.

 

[3]       ا. یزدانی چم زینی، م. رفیعی نیا، ب. موحدی و ح. صالحی، "سنتز و ارزیابی سمیت سلولی نانو الیاف شیشه­ی زیستی تهیه شده به روش الکتروریسی جهت ساخت داربست مهندسی بافت"، فرآیندهای نوین در مهندسی مواد، سال 9، صفحه 154-145، 1394.

 

[4]     Y. Zhang, M. Chen, J. Yan, Z. Ye, Y. Zhou, W. Tan & M. Lang, “Surface properties of amino-functionalized poly(ε-caprolactone) membranes and the improvement of human mesenchymal stem cell behaviorˮ, Colloid and Interface Science, Vol. 368, pp. 64–69, 2012.

 

[5]     Z. Xie, C. Lu, X. Chen, L. Chen, X. Hu, Q. Shi & X. Jing, “A facile approach to biodegradable poly(ε-caprolactone)-poly (ethylene glycol)-based polyurethanes containing pendant amino groupsˮ, European Polymer Journal, Vol. 43, pp. 2080–2087, 2007.

 

[6]     G. G. Ayala, E. D. Pace, P. Laurienzo, D. Pantalena, E. Sommab & M. R. Nobile, “Poly(ε-caprolactone) modified by functional groups: Preparation and chemical–physical investigationˮ, European Polymer Journal, Vol. 45, pp. 3217–3229, 2009.

 

[7]     Q. Guo, S. Slavov & P. J. Halley, “Phase Behavior, Crystallization, and morphology in thermosetting blends of a biodegradable poly (ethylene glycol)-type epoxy resin and poly (ε -caprolactone)ˮ, Polymer Science: Part B: Polymer Physics, Vol. 42, pp. 2833–2843, 2004.

 

[8]     J. Zhu, “Bioactive modification of poly (ethylene glycol) hydrogels for tissue engineeringˮ, Biomaterials, Vol. 31, pp. 4639-4656, 2010.

 

[9]     C. P. Jiang, Y. Y. Chen & M. F. Hsieh, “Biofabrication and in vitro study of hydroxyapatite/mPEG–PCL–mPEG scaffolds forbone tissue engineering using air pressure-aided deposition technologyˮ, Materials Science and Engineering, Vol. 33C, pp. 680–690, 2013.

 

[10] B. Chuenjitkuntaworn, W. Inrung, D. Damrongsri, K. Mekaapiruk, P. Supaphol & P. Pavasant, “Polycaprolactone/Hydroxyapatite composite scaffolds: Preparation, characterization, and in vitro and in vivo biological responses of human primary bone cellsˮ, Biomedical Material Research, Vol. 94A, pp. 241–251, 2010.

 

[11] Y. Wang, L. Liu & S. Guo, “Characterization of biodegradable and cytocompatible nano-hydroxyapatite/polycaprolactone porous scaffolds in degradation in vitroˮ, Polymer Degradation and Stability, Vol. 95, pp. 207-213, 2010.

 

[12] Y. Jiang, K. Mao, X. Cai, S. Lai & X. Chen, “Poly (ethyl glycol) Assisting water sorption enhancement of poly (ε-caprolactone) blend for drug deliveryˮ, Applications Polymer Science, VoL. 122, pp. 2309–2316, 2011.

 

[13] C. S. Cho, S. Y. Han, J. H. Ha, S. H. Kim & D. Y. Lim, “Clonazepam release from bioerodible hydrogels based on semi-interpenetrating polymer networks composed of poly ( -caprolactone) and poly (ethylene glycol) macromerˮ, International Journal of Pharmaceutics, Vol. 181, pp. 235–242, 1999.

 

[14] H. Y. Kweona, M. K. Yoob, I. K. Park, T. H. Kimb, H. C. Lee, H. S. Lee, J. S. Oh, T. Akaiked & C. S. Cho, “A novel degradable polycaprolactone networks for tissue engineeringˮ Biomaterials, Vol. 24, pp. 801–808, 2003.

 

[15] L. Cai & S. Wang, “Poly (ε-caprolactone) acrylates synthesized using a facile method for fabricating networks to achieve controllable physicochemical properties and tunable cell responsesˮ, Polymer, Vol. 51, pp. 164–177, 2010.

 

[16] L. Cai, A. S. Guinn & S. Wang, “Exposed hydroxyapatite particles on the surface of photo-crosslinked nanocomposites for promoting MC3T3 cell proliferation and differentiationˮ, Acta Biomaterialia, Vol. 7, pp. 2185–2199, 2011.

 

[17] M. G. Henry, L. Cai, X. Liu, L. Zhang, J. Dong, L. Chen, Z. Wang & S. Wang, “Roles of hydroxyapatite allocation and microgroove dimension in promoting preosteoblastic cell functions on photocured polymer nanocomposites through nuclear distribution and alignmentˮ, Langmuir, Vol. 31, pp. 2851−2860, 2015.

 

[18] M. Jaiswal, A. K. Dinda, A. Gupta & V. Koul, “Polycaprolactone diacrylate crosslinked biodegradable semi-interpenetrating networks of polyacrylamide and gelatin for controlled drug deliveryˮ, BiomedicalMaterials, Vol. 5, pp. 065014, 2010.

 

[19] N. Koupaei, A. Karkhaneh & M. Daliri Joupari, “Preparation and character­ization of (PCL-crosslinked-PEG)/hydroxyapatite as bone tissue engi­neering scaffoldsˮ, Biomed Mater Res, Vol. 103A, pp. 919-3926, 2015.

 

[20] Z. L. Mou, L. J. Zhao, Q. A. Zhang, J. Zhang & Z. Q. Zhang, “Preparation of porous PLGA/HA/collagen scaffolds with supercritical CO₂ and application in osteoblast cell cultureˮ, The Journal of Supercritical Fluids, Vol. 3, pp. 398-406, 2011.

 

[21] W. W. Thein Han & R. D. K. Misra, “Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineeringˮ, Acta Biomaterialia, Vol. 4, pp. 1182-1197, 2009.

 

[22] S. Wang, M. J. Yaszemski, J. A. Gruetzmacher & L. Lu, “Photo-crosslinked poly (ε-caprolactone fumarate) networks: roles of crystallinity and crosslinking density in determining mechanical propertiesˮ, Polymer, Vol. 49, pp. 5692-5699, 2008.

 

[23] C. P. Jiang, Y. Y. Chen & M. F. Hsieh, “Biofabrication and in vitro study of hydroxyapatite/mPEG–PCL–mPEG scaffolds forbone tissue engineering using air pressure-aided deposition technologyˮ, Materials Science and Engineering, Vol. 33C, pp. 680–690, 2013.

 

[24] M. Peter, N. S. Binulal, S. Soumya, S. V. Nair, T. Furuike, H. Tamura & R. Jayakumar, “Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applicationsˮ Carbohydrate Polymers, Vol. 79, pp. 284–289, 2010.

 

[25] Z. Li, H. R. Ramay, K. D. Hauch, D. Xiao & M. Zhang, “Chitosan–alginate hybrid scaffolds for bone tissue engineeringˮ, Biomaterials, Vol. 26, pp. 3919–3928, 2005.

 

[26] L. P. Yan, J. M. Oliveira, A. L. Oliveira, S. G. Caridade, J. F. Mano & R. L. Reis, “Macro/microporous silk fibroin scaffolds with potential for articular cartilage and meniscus tissue engineering applicationsˮ Acta biomaterialia, Vol. 1, pp. 289-301, 2012.

 

[27] Y. Wang, M. A. Rodriguez Perez, R. L. Reis & J. F. Mano, “Thermal and thermomechanical behaviour of polycaprolactone and starch/polycaprolactone blends for biomedical applicationsˮ, Macromolecular Materials and Engineering, Vol. 8, pp. 792-801, 2008.

 

[28] D. Z. Chen, C. Y. Tang, K. C. Chan, C. P. Tsui, P. H. F. Yu, M. C. P. Leung & P. S. Uskokovic, “Dynamic mechanical properties and in vitro bioactivity of PHBHV/HA nanocompositeˮ, Composites science and technology, Vol. 7, pp. 1617-1626, 2007.

 

[29] C. J. Pérez, V. A. Alvarez, I. Mondragon & A. Vazquez, “Mechanical properties of layered silicate/starch polycaprolactone blend nanocompositesˮ, Polymer International, Vol. 5, pp. 686-693, 2007.

 

[30] S. N. Nazhat, M. Kellomaki, P. Tormala, K. E. Tanner & W. Bonfield, “Dynamic mechanical characterization of biodegradable composites of hydroxyapatite and polylactidesˮ, Journal of biomedical materials research, Vol. 4, pp. 335-343, 2001.

 

[31] L. Pan, X. Pei, R. He, Q. Wan & J. Wang, “Multiwall carbon nanotubes/polycaprolactone composites for bone tissue engineering applicationˮ, Colloids and Surfaces, Biointerfaces, Vol. 93B, pp. 226– 234, 2012.

 

[32] J. Venkatesan, R. Pallela, I. Bhatnagar & S. K. Kim, “Chitosan–amylopectin/hydroxyapatite and chitosan–chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineeringˮ International journal of biological macromolecules, Vol. 5, pp. 1033-1042, 2012.