The Effect of Patulin on Some Spermatic Parameters of Human
Subject Areas : Journal of Animal Biology
Z.S. Rasouli
1
(گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران)
S.F. Siadat
2
(گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران)
M. Heydari Nasrabadi
3
(گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران)
A. Shahverdi
4
(گروه جنین شناسی، مرکز تحقیقات پزشکی تولیدمثل، پژوهشکده رویان، تهران، ایران)
Keywords: Fertility, Mycotoxin, Patulin, fragmentation,
Abstract :
Patulin is a toxin from Mycotoxins family that is produced by several species of mold especially Aspergillus, Byssochlamys and Penicillium ecspansom. The presence of Penicillium Ecspansom on apples and its juice causes concern. Patulin has teratogenic on the fetus, genetic mutagenicity and carcinogenicity effects. In this study, we have tried to investigate the effect of this toxin on human sperm and its role as a male factor in men`s infertility. The purpose of study Patulin toxin has destructive and harmful effects on human sperm DNA and its excessive amounts in fruit juices. The present study has been done on 40 samples taken from infertile patients referring to Royan Institute. Mobility, pH, morphology, viability and sperm DNA fragmentation were studied before and after the use of Patulin with 5 doses of 0.02, 0.05, 0.16, 0.33 and 0.5 micrograms per milliliter that was diluted with medium Ham`sF10. The results showed that concentrations of 0.33, 0.5 and 0.05 caused a significant decrease in sperm motility, pH and viability. The concentrations of 0.33 and 0.5 caused a significant damage on sperm DNA but have not affected morphology, the number of positive peroxidase cells and sperm count.
