The Evaluation of the Interference effect of Fumaria parviflora Ethanolic Extract and Medicine Chlorambucil on the Spermatogenesis of Male Wistar Rat

Subject Areas : Journal of Animal Biology

زهرا حبیبی ¹ (دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران)
میترا حیدری نصر آبادی ² (دانشگاه آزاد اسلامی، واحد پرند، گروه زیست شناسی، پرند، ایران)
عبدالحسین شیروی ³ (دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران)
مونا سوری ⁴ (دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران)

Received: 2014-04-07 Accepted : 2017-05-21 Published : 2011-04-21

Keywords: Spermatogenesis, Rat, ethanolic extract, Fumaria parviflura, Cholorambucil,

Abstract :

This study the effects of ethanolic extract of Fumaria with the scientific name of Fumaria parviflora that belongs to the family of Fumariacea as an anti-oxidan and interference of it with chlorambucil in testis tissue is considered. The aim of this study is to investigate the effect site of chlorambucil in testis tissue and protective role of the Fumaria ethanolic extract, against male wistar rats is grouped in six, seven group(n=7). The witness 1 group was served with only liquid oil; the witness 2 group was served only with 10 mg/kg w.b. chlorambucil. The treatment 1 group 150mg/kg w.b Fumaria ethanolic extract, the treatment groups 2, 3, 4 were served with10 mg/kg w.b. chlorambucil and 150, 250, 350mg/kgw.b. Fumaria ethanolic extract respectively Chl via gavage. After 15 days the rats should be analysed and their testis were brought out and after the morphometry studies, they were studied histologically. Germinal epithelium is considered and the number of cells were counteddistinguish that chlorambucil in witness 2 group was very effective and the size of testis and the number of sperms were greatly decreased, but in treatment groups the changes were related to dosage of Fumaria ethanolic extract.