The Effects of Hydroalcoholic Extract of Salvia officinalis on Liver Tissue and Cu, Fe, Zn and Mg Elements in Male Rats
Subject Areas : Journal of Animal Biologyشهرزاد خاکپور 1 , مریم خسروی 2 , معصومه میرزائی 3 , محیا نجاری 4
1 - دانشگاه آزاد اسلامی، واحد پزشکی تهران، گروه فیزیولوژی، تهران، ایران
2 - دانشگاه آزاد اسلامی، واحد تهران شمال، گروه زیست شناسی، تهران، ایران
3 - دانشگاه آزاد اسلامی، واحد تهران شمال، گروه زیست شناسی، تهران، ایران
4 - دانشگاه آزاد اسلامی، واحد تهران شمال، گروه زیست شناسی، تهران، ایران
Keywords: Antioxidant, Oxidative stress, Isoniazid, Free radical, Salvia officinalis,
Abstract :
The imbalance between the production of free radicals and antioxidant can be the cause of oxidative stress.Oxidative stress induces different diseases and tissue damages. Isoniazid has been used for the purpose of producing free radicals. In the present study, we investigated the effects of Salvia officinalis hydroalcoholic extract on blood elements such as Fe, Mg, Zn and Cu. In this study, 63 male rats were used and divided in 9 groups; in each group 7 rats were included. Isoniazid 50mg/kg was orally administered to rats continuously once per day for 28 days using a feeding atraumatic needle. The experimental groups received for 28 days,Salvia officinalis hydroalcoholic extract with the doses 100, 250 and 400 mg/kg intraperitoneally, and also some experimental groups were coadministered with different doses of the extract and isoniazid; the results of serum Fe, Mg, Zn and Cu were compared with the control and sham groups, as well. Also, the animal livers of all groups were separated and dissected for the histopathological studies. Zn and Cu were increased significantly in the treated-groups with Salvia officinalis, but the levels of Fe and Mg showed a significant decrease in the experimental groups treated with all the doses of Salvia officinalis hydroalcoholic extract. It seems that tannin and bitter compounds of Salvia officinalis hydroalcoholic extract are the main agents to alternate the blood element levels.
