Determination of the Relative Population of Salmonella in Poultry Gastrointestinal Tract Using PCR Method
Subject Areas : Journal of Animal Biology
علیرضا صیداوی
1
(دانشگاه آزاد اسلامی، واحد رشت، گروه علوم دامی، رشت، ایران)
Keywords: Salmonella, poultry, Intestine, semi- quantitative PCR,
Abstract :
This study was conducted in order to determining of relative population of Salmonella spp. in various parts of poultry gastrointestinal tract using semi- quantitative PCR technique. It was removed gut contents and extracted their DNA. It is used polymerase chain reaction (PCR) and semi- quantitative PCR technique for investigation on relative population of these bacteria. It was obtained a specific band for detecting of mentioned bacterium using a set specific primer and also a specific band for detecting of all bacteria. Then, it was determined relative population of Salmonella spp. relative to total gut bacteria using semi- quantitative PCR technique. Result analysis was showed Salmonella spp. consists 0.12% and 0.11% of total duodenum and jejunum bacteria respectively. Also it was showed Salmonella spp. consists 0.16% and 0.39% of total ileum and cecum bacteria respectively. Meanwhile Salmonella spp. consists 0.38%, 0.18% and 0.06% of total intestine bacteria at 4, 14 and 30 day of ages. Furthermore it was showed at 4 day of ages Salmonella spp. consists 0.28%, 0.11%, 0.16% and 0.91% of total bacteria in duodenum, jejunum, ileum and cecum respectively. Thus it can state relative population of Salmonella spp. in lower parts i.e. ileum andcecum were higher than upper parts i.e. duodenumand jejunum. Obtained results showed Salmonella spp. populations were variable in various intestine parts and correlated with functions and physicochemical conditions of intestine parts.
