Culture and proliferation of dendritic cells from mouse bone marrow progenitors
Subject Areas : Journal of Animal Biologyسمانه عرب 1 , معصومه معتمدی 2 , جمشید حاجتی 3
1 - کارشناس ارشد ایمونولوژی
2 - کارشناس ارشد ایمونولوژی دانشگاه علوم پزشکی لرستان
3 - دانشیار ایمونولوژی دانشگاه علوم پزشکی تهران
Keywords: Proliferation, maturation, Bone marrow, Cell Culture, dendritic cells,
Abstract :
Dendritic cells (DCs) are the most potent antigen-presenting cells that induce and control the immune responses. These cells have the capacity of stimulation different types immune response (type 1 or 2) in exposure to peripheral signals. The aim of this study was DCs culture, proliferation and maturation from mouse bone marrow progenitors. Bone-marrow derived DCs (BMDCs) were cultured in the presence of GM-CSF and IL-4. After 5 days, LPS and cholera toxin were added to the culture for another 2 days. Expression of surface molecules and cytokine production in BMDC culture evaluated. The result shown that LPS stimulated DCs were matured. Exposure with CT induce IL-10 and with LPS induce IL-12 production by DCs. These fundings indicate that 7 days culture of mouce bone marrow progenitors can produce professional dendritic cells and with added different components in DCs culture induce appropriate type 1 or 2 of immune responses.
