Evaluation of the Effect of Mmagnesium Oxide Nanoparticles on Gad2 Gene Expression in the Hippocampus of Male Rats in the Presence and Absence of Acute Motor Restriction Stress

Subject Areas : Journal of Animal Biology

Masoomeh Esmipoor ¹ , Zohreh Valizadeh ²

1 - Department of Biology, Dezfoul Branch, Islamic Azad University, Dezfoul, Iran
2 - Department of Nursing and Midwifery, Dezfoul Branch, Islamic Azad University, Dezfoul, Iran

Received: 2021-06-10 Accepted : 2021-08-22 Published : 2022-02-20

Keywords: Stress, Hippocampus, Magnesium Nanoxide, Gad2 gene,

Abstract :

Stress is a neurological condition that affects the nerves of all organisms by molecular processes. Magnesium oxide nanoparticles effectively reduce and relieve anxiety-like behaviors, but the molecular mechanism of this relationship has not yet been studied. Gad2 gene encodes one of the isoforms of glutamate decarboxylase, an enzyme which converts glutamic acid to GABA (gamma amino butyric acid). In the present study, the effect of magnesium oxide nanoparticles on Gad2 gene expression in the hippocampus of male rats in the presence and absence of acute motor stress was investigated. 4 groups of male Wistar rats( control, receiving salin+stress, 5mg/kg Mg nanoxide, stress+ 5mg/kg Mg nanoxide) were prepared. RNA was extracted from hippocampal tissue. Then, cDNA synthesis and real-time were performed for all groups. The results showed that Gad2 gene expression was significantly (p<0.01) increased in the presence of magnesium nanoxide, especially in the group with acute motor stress compared to other groups. According to the findings, magnesium nanoxide enhances the inhibitory nervous system by enhancing the hippocampal enzyme glutamate decarboxylase. Additional research in this area will throw more light on this molecular connection.

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