The Effect of Movento Insecticide on the Fertility Rate in Male Balb/c Mice

Subject Areas : Journal of Animal Biology

Ghodrat Ebadi Manas ¹

1 - Department of Biology, Farhangian University, 14556-889 P.Box, Tehran, Iran

Received: 2023-07-13 Accepted : 2023-08-23 Published : 2024-02-20

Keywords: Embryo, Fertility, insecticide, Movento, Male mouse,

Abstract :

Today, due to the indiscriminate use of poisons and the resistance of pests to them, new types of poisons are produced every year. Movento is the latest insecticide that is used in pistachio orchards to control the psyllid pest, whose side effects on the reproductive system have not been investigated. The aim of this study was to investigate the effect of Movento on fertility in Balb/c mice. In this study, 18 adult and healthy male Balb/c mice were prepared and kept under standard conditions. Each group consisted of 9 mice. Mice were randomly divided into experimental and control groups. The experimental group received 79 mg/kg Movento and the control group received the same amount of distilled water daily for 60 days through oral gavage. At the end of the study, the mice were euthanized by injecting a mixture of xylazine and ketamine. Epididymal tail of the testes of mice was separated and placed in HTF culture medium. The parameters of sperm count, sperm viability, DNA damage of sperm, immature sperms and fertilization ability of sperms and blastocyst were evaluated. The data obtained from the study were analyzed by ANOVA method. The findings showed that sperm viability, the percentage of fertilized oocytes, two-celled and four-celled embryos, and the number of blastocysts were significantly reduced in the experimental group compared to the control. Also, sperms count in the experimental group compared to the control group decreased but was not significant. However, immature sperms and DNA damage of sperm in the experimental group was increased compared to the control group. Therefore, it can be concluded that Movento poison reduces fertility in mice by DNA damage of sperm and chromatin.

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