Effect of ZnFe2O4@Ag Nanocomposite Biosynthesized by Chlorella vulgaris on the Expression of P53, Caspase 9, and CAD Genes in Breast Cancer Cell Line

Subject Areas : Journal of Animal Biology

Ayda Mohammad Amoie ¹ , Vajiheh Zarrinpour ² , Seyed Ataollah Sadat Shandiz ³ , Ali Salehzadeh ⁴

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

Received: 2022-07-26 Accepted : 2022-09-24 Published : 2023-05-22

Keywords: breast cancer, nanocomposite, Apoptosis, Caspase,

Abstract :

Regarding the increasing trend of the morbidity and mortality of breast cancer, the use of nanotechnology products in breast cancer treatment has gained interest. The current work aims to synthesize ZnFe2O4@Ag nanocomposite using the Chlorella vulgaris extract, assessment of its anticancer potential on breast cancer cells, and evaluate the effect of the nanocomposite on the expression of apoptotic genes. Anticancer potential and lethal dose 50% (IC50) of ZnFe2O4@Ag were determined using the MTT assay and real-time PCR was employed to evaluate the expression of p53, caspase 9, and CAD genes. According to the results, at concentrations greater than 15.62 µg/mL, ZnFe2O4@Ag considerably decreased the viability of breast cancer cells and IC50 was determined 28µg/mL. Moreover, the relative expression of the p53, caspase 9, and CAD genes was significantly increased by 2.22, 1.98, and 5.96 folds, respectively after exposure to the nanocomposite. Owing to the presence of silver and zinc, it seems that ZnFe2O4@Ag generates reactive oxygen species that could damage cell components and subsequently induce cell apoptosis.

References:

1. Abbasian A.R., Shafiee Afarani M. 2019. One-step solution combustion synthesis and characterization of ZnFe₂O₄ and ZnFe1. 6O4 nanoparticles. Applied Physics A, 125(10):1-2.


2. Akhtar M.J., Ahamed M., Kumar S., Khan M.M., Ahmad J., Alrokayan S.A. 2012. Zinc oxide nanoparticles selectively induce apoptosis in human cancer cells through reactive oxygen species. International Journal of Nanomedicine,7:


3. Avval Z.M., Malekpour L., Raeisi F., Babapoor A., Mousavi S.M., Hashemi S.A., Salari M. 2020. Inroduction of magnetic and supermagnetic nanoparticles in new approach of targeting drug delivery and cancer therapy application. Drug Metabolism Reviews,52(1):157-184.


4. Awasthi R., Roseblade A., Hansbro P.M., Rathbone M.J, Dua K. Bebawy M. 2018. Nanoparticles in cancer treatment: opportunities and obstacles. Current Drug Targets, 19(14): 1696-1709.


5. Banu H., Renuka N., Faheem S.M., Ismail R., Singh V., Saadatmand Z., Khan S.S., Narayanan K., Raheem A., Premkumar K., Vasanthakumar G. 2018. Gold and silver nanoparticles biomimetically synthesized using date palm pollen extract-induce apoptosis and regulate p53 and Bcl-2 expression in human breast adenocarcinoma cells.Biological Trace Element Research, 186(1):122-134.


6. Bisht G., Rayamajhi S. 2016. ZnO nanoparticles: a promising anticancer agent. 3(2016): 3-9.


7. Gour A., Jain N.K. 2019. Advances in green synthesis of nanoparticles. Artificial Cells, Nanomedicine, and Biotechnology,47(1):844-851.


8. Khan F., Shahid A., Zhu H., Wang N., Javed M.R., Ahmad N., Xu J., Alam M.A., Mehmood M.A. 2022. Prospects of algae-based green synthesis of nanoparticles for environmental applications. Chemosphere,293:


9. Kuida K. 2000. Caspase-9. The International Journal of Biochemistry and Cell Biology,32(2):121-124.


10. Mladenova B., Milusheva Y., Karsheva M., Hinkov I., Stankulov T., Borisov G., Boukoureshtlieva R. 2018. Nanosized Ag particles as catalyst in gas-diffusion electrodes for ORR. Bulgarian Chemistry Communication, 50:114-118.


11. Norbury C.J., Zhivotovsky B. 2004. DNA damage-inducedapoptosis. Oncogene, 23(16):2797-2808.


12. Pfaffl M.W. 2001. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Research, 29(9):e45-e45.


13. Salehzadeh A., Naeemi A.S., Khaknezhad L., Moradi-Shoeili Z., Shandiz S.A.S. 2019. Fe₃O₄/Ag nanocomposite biosynthesized using Spirulina platensis extract and its enhanced anticancer efficiency. IET Nanobiotechnology, 13(7): 766-770.


14. Sarala E., Madhukara Naik M., Vinuth M., Rami Reddy Y.V., Sujatha H.R. 2020. Green synthesis of Lawsonia inermis-mediated zinc ferrite nanoparticles for magnetic studies and anticancer activity against breast cancer (MCF-7) cell lines. Journal of Materials Science: Materials in Electronics, 31(11):8589-8596.


15. Shen Y., White E. 2001. p53-dependent apoptosis pathways. Advances in Cancer Research, 82:55-84.


16. Shokoofeh N., Moradi-Shoeili Z., Naeemi A.S., Jalali A., Hedayati M., Salehzadeh A. 2019. Biosynthesis of Fe₃O ₄@Ag nanocomposite and evaluation of its performance on expression of norA and norB efflux pump genes in ciprofloxacin-resistant Staphylococcus aureus. Biological Trace Element Research, 191(2):522-530.


17. Sofi M.A., Sunitha S., Sofi M.A., Pasha S.K., Choi D. 2021. An overview of antimicrobial and anticancer potential of silver nanoparticles. Journal of King Saud University-Science, 3032:101791.


18. Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., Bray F. 2021. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a Cancer Journal for Clinicians,71(3):209-249.


19. Widłak P. 2000. The DFF40/CAD endonuclease and its role in apoptosis. Acta Biochimica Polonica,47(4):1037-1044.


20. Yu W., Hu C., Gao H. 2021. Advances of nanomedicines in breast cancer metastasis treatment targeting different metastatic stages. Advanced Drug Delivery Reviews, 178:


21. Zhao Y., Guo C., Wang L., Wang S., Li X., Jiang B., Wu N., Guo S., Zhang R., Liu K., Shi D. 2017. A novel fluorinated thiosemicarbazone derivative-2-(3, 4-difluorobenzylidene) hydrazine carbothio amide induces apoptosis in human A549 lung cancer cells via ROS-mediated mitochondria-dependent pathway. Biochemical and Biophysical Research Communications, 491(1):65-71.