Effect of ZnFe2O4@Ag Nanocomposite Biosynthesized by Chlorella vulgaris on the Expression of P53, Caspase 9, and CAD Genes in Breast Cancer Cell Line

Subject Areas : Journal of Animal Biology

Ayda Mohammad Amoie ¹ (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Vajiheh Zarrinpour ² (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Seyed Ataollah Sadat Shandiz ³ (Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
Ali Salehzadeh ⁴ (Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran)

Received: 2022-07-26 Accepted : 2022-09-24 Published : 2023-05-22

Keywords: breast cancer, nanocomposite, Apoptosis, Caspase,

Abstract :

Regarding the increasing trend of the morbidity and mortality of breast cancer, the use of nanotechnology products in breast cancer treatment has gained interest. The current work aims to synthesize ZnFe2O4@Ag nanocomposite using the Chlorella vulgaris extract, assessment of its anticancer potential on breast cancer cells, and evaluate the effect of the nanocomposite on the expression of apoptotic genes. Anticancer potential and lethal dose 50% (IC50) of ZnFe2O4@Ag were determined using the MTT assay and real-time PCR was employed to evaluate the expression of p53, caspase 9, and CAD genes. According to the results, at concentrations greater than 15.62 µg/mL, ZnFe2O4@Ag considerably decreased the viability of breast cancer cells and IC50 was determined 28µg/mL. Moreover, the relative expression of the p53, caspase 9, and CAD genes was significantly increased by 2.22, 1.98, and 5.96 folds, respectively after exposure to the nanocomposite. Owing to the presence of silver and zinc, it seems that ZnFe2O4@Ag generates reactive oxygen species that could damage cell components and subsequently induce cell apoptosis.

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