The Effect of 8 Weeks of High Intensity Interval Training on ICAM and CRP Levels in the Left Ventricle of Obese Rats

Subject Areas : Journal of Animal Biology

Rasol Fotohi ardahaee ¹ , neda aghaee ² , reza rezaeeshirazi ³ , mojtaba izadi ⁴

1 - Department of Exercise Physiology, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 - Department of Exercise Physiology, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
3 - Department of Exercise Physiology, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
4 - Department of Exercise Physiology, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

Received: 2024-01-09 Accepted : 2024-06-26 Published : 2024-08-06

Keywords: Obesity, High intensity interval training, Gene expression, ICAM, CRP, Obese rats,

Abstract :

The role of obesity in chronic diseases such as type 2 diabetes and cardiovascular diseases is well known. This study was conducted to determine the effect of eight weeks of intense intermittent exercise on the expression of CRP and ICAM genes in the left ventricle of obese rats. The statistical population of the current study consists of male Wistar rats from the Pasteur Institute in Tehran. 21 male Wistar rats were selected and after induction of obesity, they were divided into three groups: normal, obese control, and periodic obese. Six weeks of a high-fat diet was used to induce obesity. The rats in the exercise group performed an 8-week interval training program in the form of interval runs on the treadmill. The normal and obese control group did not participate in the exercise program. 48 hours after the last training session, dissection was performed. The independent t-test was used to compare the pre-tests and the correlated t-test was used to determine the level of intra-group changes at a significant level. Intermittent training significantly reduced the expression of ICAM compared to the obese control group (p < 0.0001) so that its levels were close to the normal group. In other words, there was no significant difference in the expression of ICAM between the intermittently obese group and the normal group. The expression of CRP decreased significantly due to intermittent exercise compared to the obese control group, so its levels were close to the normal group. There was no significant difference in the expression of CRP between the intermittently obese group and the normal group. Intense intermittent exercise decreased ICAM and CRP compared to the control group. Probably, intermittent training inhibits the release of inflammatory mediators from fat tissue by increasing the activity of the sympathetic system and increasing anti-inflammatory cytokines, and subsequently, the concentration of cell adhesion molecules decreases.

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