analysis of Association among ISSR Markers and Important Traits in Different Soybean Cultivars by Regression Method
Subject Areas :zahra malekmohamadi 1 , hosein sabori 2 , abas beyabani 3 , ebrahim hezarjaribi 4 , ahmadreza dadras 5
1 - gonbad city, univercity agricultury
2 - associated prof of college of Agriculture Science and Natural Resource, Gonbad Respectively
3 - associated prof of college of Agriculture Science and Natural Resource, Gonbad Respectively
4 - Agricultural and Natural Research Center of Golestan Province
5 - PhD student in Plant Breeding, University of Guilan
Keywords: coefficient of determination, . Marker Index, Polymorphism information content, higher resolution, breeding programs,
Abstract :
.One of the big advances in identifying markers is the identification of continuous markers with agronomic traits, as well as the value of markers in the analysis of heritability of traits in crops is specified. In this study was assessed, the relationship between agronomic traits and molecular marker ISSR in different genotypes of soybean. The genetic diversity different genotypes of soybean by using the 10 ISSR primer showed that Among 68 amplified fragment of cultivars were generated, 34 fragments were ploy morphic. PIC values varied between 0.147 to 0.058 for primers. Maximum level of marker index obtained for primer PRI-6 with value of 8.39 which indicating a higher resolution of this primer than the other. Association analysis was performed using structure matrix and statistical models of GLM and MLM by using TASSEL software for 21 variables. The MLM model in 5 percent probability level identified 6 markers related to evaluated traits. According to the results of Association analysis the highest of coefficient determination was for ISSR9-2 with 0.21 percent explanation of variation of number main branch. A total of, due to the high genetic diversity statistics, the ISSR10-1, ISSR2-3 and ISSR2-4 markers can be used to other breeding programs
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