Comparison macroscopic & microscopic of anti-adhesion properties of honey and corticosteroids following experimental surgery in rabbit abdominal cavity

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Pejman Nazem zomorodi ¹ , Kimia sattari ²

1 - دانشکده دامپزشکی- گروه علوم درمانگاهی
2 - Student in veterinary medicine, Semnan University, Semnan, Iran.

Received: 2022-02-22 Accepted : 2022-04-25 Published : 2022-02-20

Keywords: Rabbit, Honey, Adhesion, Corticosteroids, Peritoneum,

Abstract :

Background : This study compares the effects of intraperitoneal use of honey and dexamethasone to prevent the formation of intra-abdominal adhesions after surgery in rabbits.Methods: A total of 24 New Zealand white rabbits underwent laparotomy. To create adhesions in the abdomen, the serous surface of the descending colon was rubbed 100 times with a dry tampon so that patchy spots appeared on its surface. Then, 10 parallel incisions with a length of 3 cm were made on the left wall of the abdomen. At this stage, the rabbits were divided into four equal groups of 6.In group 1 (control)an amount of 5 ml of normal saline - group 2 (dexamethasone)an amount of 5.4 ml of normal saline + 0.6 mg / kg dexamethasone (0.5 ml) - in group 3 (honey)an amount of 5 ml of honey and in group 4 (Honey - Dexamethasone) 4.5 ml of honey + 0.6 mg / kg dexamethasone (0.5 ml) was poured into the abdominal cavity.The rabbit's abdominal muscles and skin were then sewn normally and cared for 21 days. After 21 days, all rabbits were sacrificed painlessly by anesthesia injection and after necropsy the degree of intra-abdominal adhesions was determined macroscopically. Then, for histopathological examinations and determination of fibrosis, a tissue sample was taken from the abdominal wall and colon.Results: The control group showed a high degree of adhesion between the abdominal wall and organs as well as large masses of adhesion. In the dexamethasone group, the adhesions were thick and narrow bands between the internal organs of the abdomen. In the honey and honey-dexamethasone groups, very little adhesions (up to two rabbits) were observed in the form of very thin and limited bands.Conclusion: The results of this study show that intraperitoneal use of honey and dexamethasone reduces intra-abdominal adhesions after surgery in rabbits. But honey is more effective than dexamethasone to prevent adhesions and reduce peritoneal inflammatory reactions as well as wound healing.

References:

- Ahmed,  A., Sulaiman, S., Amin Baig, A., et al. ( 2018 ) Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action. Hindawi. Oxidative Med and Cellular Longevity. 19.

- Aljady, A.M., Kamarudin, M.Y., Jamal, A.M., et al. ( 2000 ) Biochemical study on the efficacy of Malaysian honey on inflicted wounds:An animal model. Medi J of Islamic Acad of Sci, 13(3):125-132.

- Avsar, F.M., Sahin, M., Aksoy, F., et al.( 2001 ) Effects of  diphenhydramine HCL and methylprednisolone  in the prevention of abdominal adhesions. Am J Surg, 181(6):512-515.

- Beykaya, M., (2021 ) Determination of physicochemical properties of raw honey samples. Progress in Nutrition, 23(1)

- Blauer, K.L., Collins, R.L., ( 1998 ) The effect of intraperitoneal progesterone on postoperative adhesion formation in rabbit. Fertil Steril, 49:144-149.

- Brudzynski, K., ( 2021 ) Honey as an Ecological Reservoir of Antibacterial Compounds Produced by Antagonistic Microbial Interactions in Plant Nectars, Honey and Honey Bee. Antibiotics,10: 551.

- CharboneauID, A., Delaney, J., Beilman, G., ( 2018 ) Fucoidans inhibit the formation of postoperative abdominal adhesions in a rat mode. Plos One, 10:1-13.

- Diamond, M., & Burns, E., Accomando, B., ( 2012 ) Seprafilm® adhesion barrier: (2) a review of the clinical literature on intraabdominal use. Gynecol Surg , 9:247–257.

- Du, X.H., Liu, J.Q., Xin, K., ( 2015 ) Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats. Brazilian J of Med and Biol Res, 48(4): 344-348

- Emre, A., Akin, M., Isikgonul, I., et al.  ( 2009 ) Comparison of intraperitoneal honey and sodium hyaluronate – carboxymethylcellulose (Seprafilm) for the prevention of postoperative intra-abdominal adhesions. Clinics, 64(4):363-368.

- Guzmán-Valdivia Gómez, G.,  Tena-Betancourt, E., Martínez de Alva Coria, P., ( 2018 ) Postoperative Abdominal Adhesions: Pathogenesis and Current Preventive Techniques, World J Surg Surgical Res. 1: 1008.

- Javanmardi, S., Golmohammadi, S., Mazaheri-khamene, R., ( 2017 ) Evaluation of Silymarin effects on post-operative peritoneal adhesion in Rats, The J of Urmia Uni of Medi Sci, 28(8)

- Leon De Wilde, R., Devassy, R.,  Broek,  R., et al.( 2022 ) The Future of Adhesion Prophylaxis Trials in Abdominal Surgery: An Expert Global Consensus. J. Clin. Med, 11: 1476.

- Miguel, M.G., Antunes, M.D., Faleiro, M.L. ( 2017 ) Honey as a Complementary Medicine. Integrative Medicine Insights. 12: 1-15.

- Purandare, N., Kramer, K., Minchella, N., ( 2022 ) Intraperitoneal Triamcinolone Reduces Postoperative Adhesions, Possibly through Alteration of Mitochondrial Function, J. Clin. Med, 11: 301.

- Ranneh, Y., Akim, A., Hamid, H., et al. ( 2021 )  Honey and its nutritional and antiinflammatory value. BMC Complementary Med and Therapies, 21:30.

 - Rodgers, K.E.,  Girgis, W., Amand, K.,  (1998 ) Reduction of adhesion formation by intraperitoneal administration of various anti-inflammatory agents. J Invest Surg, 11:327-339.

- Šedik,  P.,  Predanócyová, K.,  Horská, E., et al. ( 2020 ) The Antimicrobial activity of polyfloral honey and its awareness among urbanconsumers in slovakia,  Slovak J of Food Sci. 15: 467-474.

-Singer, E.R., Livesey, M.A., Barker, I.K., et al. (1996 ) Utilization of the serosal scarification model of postoperative intestinal adhesion formation of  investigate potential adhesion-preventing substances in the rabbit. Can J Vet Res, 60(4):305-311.