Invitro antagonistic effects of normal vaginal Lactobacilli isolated from healthy bitches on some pathogens related to bitch endometritis in comparison with some commonly used antibiotics

Subject Areas : clinical veterinary science

مهرداد Jabarzadeh ¹ , o. ataie amarloie ² , f. moosakhani ³

1 - Department of Clinical Sciences, College of veterinary medicine, Karaj branch , Islamic Azad University, Karaj, Iran
2 - Department of Clinical Sciences, College of veterinary medicine, Karaj branch , Islamic Azad University, Karaj, Iran
3 - Department of Microbiology, College of veterinary medicine, Karaj branch, Islamic Azad University, Karaj, Iran.

Received: 2016-12-18 Accepted : 2016-12-18 Published : 2014-09-23

Keywords: Lactobacilli, Dog, Endometritis,

Abstract :

Nowadays the uterine infections are considered as one of the principal disorders of bitch’s reproductive system. Forthis reason, the utilization of lactobacillus spp. as probiotic and aimed at substituting in the uterus as an inhibiting factoragainst the uterine infections due to the production of metabolites such as hydrogen peroxide and lactic acid be usefuland should be investigated (13). For this reason, in order to isolate the lactobacillus spp. from the vaginas of healthybitches beings, an attempt was made to obtain specimens using double guard swab under sterile conditions from twentyhealthy bitches which had been primarily confrmed through radiograph, ultrasonography and clinical examinations.Subsequent to the acquisition of specimens, the swabs were immediately placed in MRS-broth medium and aftertransferring to laboratory they were incubated frstly for 48 to 72 hours in anaerobic condition under 37ºc. Thereafter,from the specimens grown on MRS-agar, cultures were prepared and further incubated under above conditions formore 48 to72 hours. Utilizing direct observation, biochemical tests and ultimately PCR and sequencing the genus oflactobacillus spp. was discerned from the colonies grown on the surface. Following this step, the lactobacillus spp.were subjected to isolation and propagation. On the other hand, in a bid to test the effect of isolated lactobacillus onregular pathogens occurring in uterine infections, a certain number of afflicted bitches beings referred to medical clinicsexisting all over Alborz province were selected for obtaining specimens of uterine secretions. In continuation, the degreeof created inhibition zone resulting from separate proximity of lactobacilli and antibiogram discs with main isolatedpathogens including Staphylococcus intermedius, E. coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcusaureus and Trueperella were compared. The fndings demonstrated that the Lactobacilli had a signifcant effect ona multitude of pathogenic bacteria for the creation of inhibition zone from growth in laboratory conditions. Most ofisolated pathogens (83.3%) in the vicinity of 2.5µl of fresh culture medium of Lactobacillus showed susceptibility whichindicates a signifcant difference in comparison with susceptibility of all antibiogram discs under investigation (34.16%).In the light of acquired results in laboratory conditions (in vitro), it seems that the application of Lactobacillus spp. in bitchvagina as a preventive factor from uterine infections or as a factor for mitigating the disposition to such infections shouldalso be surveyed under clinical conditions which might possibly be a suitable replacement for antibiotic treatments.