Effect of Oleuropein on blood glucose and gene expression of pyruvate kinase in Streptozotocin-induced diabetic male rats
Effect of oleuropein on blood glucose and pyruvate kinase gene in diabetic rats
Subject Areas : Plasma biomarkers
Akram Asadi
1
(de)
مریم رفیعی راد
2
(گروه زیست شناسی دانشگاه آزاد اسلامی واحد ایذه)
Ameneh Javid
3
(department biology)
Keywords: Diabetic, oleuropein, pyruvate kinase, rat,
Abstract :
Background & Aim: Considering the antioxidant properties of oleuropein and the role of antioxidants in improving diabetes, this study was conducted with the aim of investigating the effect of oleuropein on blood glucose levels and pyruvate kinase gene expression in diabetic male rats. Materials & Methods: 40 rats aged 2-3 months (weighing 200-250 grams) were divided into four groups of ten. By injecting streptozotocin (60 mg/kg), type 1 diabetes was induced in male rats. After intraperitoneal injection of STZ, animals with blood glucose above 200 mg/kg were considered diabetic. The control groups and the diabetic group only received saline and the treated diabetic groups received 10, 20 mg/kg oleuropein daily by gavage for four weeks. At the end of the experiment period, all the rats were dissected after anesthesia with ether by drawing blood from the heart and liver tissue was isolated and used to check the expression of pyruvate kinase gene by Real-time PCR method. Data analysis was performed using Tukey's test at P<0.05 level. Results: The results showed that in the diabetic group receiving doses of 10 and 20 mg/kg of oleuropein, the blood glucose level decreased significantly compared to the diabetic group. Examining the expression of pyruvate kinase gene also showed that there was a decrease in the diabetic group compared to the healthy control group, but this decrease was not statistically significant. On the other hand, in the diabetic group treated with 20 and 10 mg/kg doses of oleuropein, pyruvate kinase gene expression increased significantly compared to the diabetic group. Conclusion: The results of the present study showed that the administration of oleuropein has been able to reduce blood glucose by changing the expression of the pyruvate kinase gene.
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