The reaction of flue-cured tobacco genotypes to root- knot nematode (Meloidogyne incognita)

Subject Areas : Agroecology Journal

Afshin Sajjadi ¹ (Master of plant pathology Department ofPlant Pathology TirtashReaserch and Education Center Behshahr, Iran)
Hoda assemi ² (PhD in Entomology Department ofPlant Pathology TirtashReaserch and Education Center Behshahr, Iran)

Received: 2012-10-28 Accepted : 2015-02-01 Published : 2014-08-23

Keywords: Resistance, Bel 61-10, Coker 176, race, Tolerance, Suscebtible,

Abstract :

Root knot causing by nematodes is a major disease of tobacco in all tobacco-producing countries in subtropical and tropical zones. Resistant cultivars implementation is the preferable economic management method. Nematicides are expensive and may pollute the environment. Therefore, this study evaluated reaction of 100 flue-cured tobacco genotypes to Meloidogyne incognita based on completely randomized design with three replications in greenhouse at 25 ± 3oC of Tirtash Research and Education Center in 2010-2011 years. Each tobacco seedling was inoculated with 2000 eggs and second stage juveniles in 1.5 kg pot soil. After 60-70 days they have evaluated according to gall index, reproduction factors, number of egg masses and average of eggs per egg mass. Results of variance analysis showed that there were significant differences among cultivars. Genotypes of Coker 176, Mac Nair 944, S 392-3 S, TL33 and Bel 61-10 had the lowest gall index and reproduction factors. Eighteen susceptible and resistance genotypes were selected based on results of 2010 year and based on completely randomized design with five replications. The experiment was conducted in Tirtash Research and Education Center in 2011 year. Results of variance analysis showed that there were significant differences among the cultivars. Genotypes of Bel 61-10 with the lowest of gall index, reproduction factors, number of egg masses and average of eggs per egg mass, respectively 2, 1.1, 38.2 and 357 and Coker 176 by 1.6, 1.18, 38 and 354 as resistant genotypes were introduced. Genotype of Bel 61-10 as resistant parents in breeding work will be used in the future. Cultivar of Coker 176 for cultivation in the affected tobacco growing areas to root knot nematode is recommending.

References:

1. Ahmadi R, Mortazavi Bac A (2005) Reaction of some tomato cultivars to root – knot nematode (Meloidogyne javanica). Iranian Journal of Plant Pathology 41 (3): 403-414.[In Persian with English Abstract].


2. Botha M S ( 2001) Progress with the establishment of root – knot nematode (Meloidogyne spp.) resistance in tobacco germplasm. Coresta Meet 364-366.


3. Canto-Saenz M (1985) The nature of resistance to Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 In: Sasser JNand Carter CC (Eds). An advanced treatise on Meloidogyne Vol. 1 Biology and Control. North Carolina State University Graphics. Raleigh, North Carolina. Pp. 225-231.


4. Choi Y E (1978) Studies on root-knot nematodes in Korea. The Kasetsart Journal 12(1): 31-35.


5. Di Vito N, Lombardi D A, Zaccheo G, Catalano F (1998) Response of lines and cultivars of tobacco and Nicotiana species to Italian populations of Meloidogyn species. Nematology Mediterrane 26: 271-274.


6. Dropkin V (1969) The necrotic reaction of tomato and other plants resistance to Meloidogyne reversed by temperature. Phytopathology 59: 1632-1637.


7. Fortnum B A, Bremm F (2008) Collaborative study on nematodes, report. Coresta Meet. 147 pp.


8. Gilbert J C, Guire D C (1956) Inheritance of resistance to severe root-knot from Meloidogyne incognita in commertial type tomatoes. American Society Horticultural Science. 68: 437-442.


9.   Honarnejad R, Shoai Deilami M (1998) Genetic of resistance of tobacco germplasm to Meloidogyne incognita. Coresta Meet. P 144.

10  Honarnejad R (2000) investigation on genetics of resistance to root-knot nematode (Meloidogyne incognita) in tobacco(Nicotiana tabacum). Contributions to Tobacco Research. 19(1): 17-23.

10. Lucas G B (1975) Disease of Tobacco,3^rd edition, Biological Associates consulting, Releight, North Carolina 621pp.

11. NG' Ambi T B S, Rufty R C, Barker K R (1999) Genetic Analysis of Meloidogyne arenaria Race 1 resistance in tobacco. Plant Disease 83:9. 810-813.

12. NG' Ambi T B S, Rufty R C, Barker K R (1999) Response of root-knot  resistance tobacco to concomitant populations of Meloidogyn species. Plant Disease 79:10. 1008-1013.

13. NG' Ambi T B S, Rufty R C, Barker K R, Melton T A (1999) Identification of sources of resistance to four species of root-knot nematodes in tobacco. Journal of Nematology. 31:3. 272-282.

14. Oostenbrink M (1966) Major characteristics of the relation between nematodes and plants. Medd. Land bouwhogesch. Wageningen. 66:4

15. Sajjadi S A, Khateri H, Hoseini S A, Moarefzadeh N, Najafi M R, Assemi H, Rahbari A (2006) Studying

1. 16.  the reaction of tobacco cultivars to Root-Knot Nematode in Golestan province. Proceedings of the 17^th Iranian plant protection congress. 509 pp.[In Persian with English Abstract].

17. Sajjadi S A, Hosseininejade S A, Assemi H, Amraae V (2008) Chemical control of  tobacco root knot nematode(M. incognita). Coresta Meet. P 134. (Abst.).

18. Starr J L, Bridge J, Cook R (2002) Resistance to plant-parasitic nematodes: History, current use and future potential . Starr J L, Bridge J, Cook R (eds). Plant resistance to parasitic nematodes. CABI Publishing, Wallingford, UK. Pp: 1-22.

1. 19.  Taylor A L, Sasser J N (1978) Biology, identification and control of root knot nematode (Meloidogyne spp.). North Carolina State University. 111pp.

20. Yi Y-H, Rufty R C, Wernsman E A, Conkling M C (1998) Mapping the root-knot nematode resistance gen(RK) in tobacco with RAPD markers. Plant Disease 82: 1319-1322.

Zeck W M (1971) A rating scheme for field evaluation of root knot nematode infestations. Pflanzenschutz-  Nachrichten. Bayer AG. 24:141-144.