In vitro pathogenicity of fungi Pochonia chlamydosporia var. chlamydosporia isolates on root-knot nematode Meloidogyne javanica

Subject Areas : Agroecology Journal

Seyyed Mohammad Reza Moosavi ¹ , Seddigeh Fatemi ² , Rasoul Zare ³ , Hamid reza Zamanizadeh ⁴

1 - Ph.D. student of Islamic Azad University, Science and Research Branch
2 - Scientific Board of Nematology Department, Iranian Research Institute of Plant Protection
3 - Scientific Board of Botany Department, Iranian Research Institute of Plant Protection
4 - Scientific Board of Islamic Azad University, Science and Research Branch

Received: 2007-05-10 Accepted : 2007-10-06 Published : 2007-09-23

Keywords: Biological control, root-knot nematode, Meloidogyne javanica, Pochonia chlamydosporia var. chlamydosporia, pathogenicity test,

Abstract :

Biological control is a safe tool for plant pest and disease management and numerous efforts have been done to introduce biological control agents for nematodes controlling. Pochonia chlamydosporia var. chlamydosporia is one of promising biological control agents with high potential to reduce root-knot nematode (RKN) and cyst nematode populations. In this experiment, 128 and 18 soil samples were collected during 2004 to 2006 from fields infected by cyst nematodes and RKN in Fars province of Iran, respectively. Media for selective isolation were prepared on the basis of CMA and Shrimp-Agar. Pathogenicity test was done on 13 isolates of P. chlamydosporia var. chlamydosporia obtained from CBS collection and Iran. Then the numbers of infected immatures, matures, empty and uninfected eggs were recorded. The mycelium of all tested isolates penetrated in eggs of M. javanica in a range of 39.85% to 90.17%. There was a significant difference in the ability of isolates in parasitizing eggs (P<0.0001). Mentioned isolates were placed in 3 different groups using Tukey test. 10 isolates could invade mature eggs in a low range and there was no consistent correlation between the percent of infected eggs and hatched juveniles.

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