Synthesis and Evaluation of Antibacterial Activity of Silver-Doped Titania Nanoparticles as an Antibacterial Additive to Dental Materials

Subject Areas : journal of New Materials

N. Bahremandi Tolou ¹ , M.H. Fathi ² , A. Monshi ³ , V.S. Mortazavi ⁴ , F. Shirani ⁵ , M. Mohammadi Sichani ⁶

1 - کارشناس ارشد مهندسی مواد، دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران.
2 - استاد، گروه پژوهشی بیومواد، دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران.
3 - استاد، دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران.
4 - استاد، مرکز تحقیقات دندانپزشکی پروفسور ترابی‌نژاد و گروه ترمیمی و زیبایی دانشکده دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران.
5 - دانشیار، مرکز تحقیقات دندانپزشکی پروفسور ترابی‌نژاد و گروه ترمیمی و زیبایی دانشکده دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران.
6 - مربی، دانشگاه آزاد اسلامی، واحد فلاورجان، گروه میکروبیولوژی، اصفهان، ایران.

Received: 2013-09-16 Accepted : 2013-09-16 Published : 2013-09-23

Keywords: Nanoparticles of Titania, Silver, Antibacterial Property,

Abstract :

In this study, antibacterial behavior of nanoparticles of titania and nanoparticles of silver-doped titania were evaluated against Streptococcus mutans and were compared with three pathogen bacteria. Initially, the nanoparticles of titania and nanoparticles of silver-doped titania were synthesized by sol-gel method. The powders were characterized by X-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Energy-Dispersive Spectroscopy (EDS) techniques. Then, Agar dilution was used to evaluate antibacterial properties of silver- doped titania nanoparticles against Streptococcus mutans and three types of pathogen bacteria. Phase structure evaluation showed anatase phase of titania in all silver-dopped titania nanoparticles. The particle size of titania and silver particles were determined 30 and 15 nanometers, respectively. The antibacterial results showed that Minimum Inhibition Concentration (MIC) of 3 mg/ml for silver-doped titania nanoparticles with different percentages of silver could inhibit bacteria growth, while nanoparticles of titania in fluorescent light indicated reducing growth of bacteria. Furthermore, MIC of 4 mg/ml was achieved for silver-doped titania nanoparticles with 5 mole percent sliver against the other pathogen bacteria and for other specimens observed growth of bacteria. These results showed that silver could increase antibacterial properties of titania. This is due to presence of silver nanoparticles and also the effect of silver on the photocatalyst properties increased antibacterial properties.

References:

1- M. Guzman, J. Dille, S. Godet, "Synthesis

and Antibacterial Activity of Silver

Nanoparticles Against Gram-Positive and

Gram-Negative Bacteria", Nanomedicine:

Nanotechnology, Biology, and Medicine, Vol.

8, p.p 37–45, 2012.

2- E. Pipelzadeh, A.A. Babaluo, M. Haghighi,

1. Tavakoli, M. Valizadeh Derakhshan, and A.

Karimzadeh Behnami, “Silver Doping on TiO2

Nanoparticles Using a Sacrificial Acid and its

Photocatalytic Performance Under Medium

Pressure Mercury UV Lamp”, Chemical

Engineering Journal, Vol. 155, pp. 660–665,

2009.

3- A.L Linsebigler, G. Lu, and J.T. Yates,

“Photocatalysis on TiOn Surfaces: Principles,

Mechanisms, and Selected Results”, Chemical.

Review, Vol. 95, pp. 735-758, 1995.

4- P. Chin, Kinetics of Photocatalytic

Degradation Using Titanium Dioxide Films,

ProQuest, p. 2-7, 2008.

5- C. Yi, P. Xiao-Yan, M. Xue-Ming, and Hui,

Z.,” Study on Photocatalysis Properties of

Nanocrystalline Titanium Dioxide”, Journal of

Shanghai University, Vol. 6, pp. 240-243,

2002.

6- A. Zaleska, ” Characteristic of Doped-TiO2

Photocatalists”, Physicochemical Problems of

Mineral Processing, Vol. 42, pp. 211-222, 2008.

7- D. Beydoun, R. Amal, G. Low, and S.

Evoy” Role of Nanoparticles in

Photocatalysis”, Journal of Nanoparticle

Research, Vol. 1, pp. 439 – 458, 1999.

8- A. Alem, and H. Sarpoolaky” The Effect of

Silver Doping on Photocatalytic Properties of

Titania Multilayer Membranes”, Solid State

Sciences, Vol. 12, pp. 1469-1472, 2010.

9 -م. مختاری مهر، م. ح. شریعت و م. پاک شیر، "بررسی

خاصیت فتوکاتالیستی لایه نازک2 TiO doped V تهیه

شده با روش سل-ژل"، مجله مواد نوین، جلد2 ،شماره 3،ص

.1391 بهار، 21 -26

10- S. Sivakumar, P. Krishna Pillai, P.

Mukundan, and K.G.K. Warrier, ” Sol–gel

Synthesis of Nanosized Anatase from Titanyl

Sulfate”, Materials Letters, Vol. 57, pp. 330–

335, 2002.

11- M.K.Seery, R. George, P. Floris, and S.C.

Pillai., ”Silver Doped Titanium Dioxide

Nanomaterials for Eenhanced Visible Light

Photocatalysis”, Journal of Photochemistry and

Photobiology,Vol. 189, pp. 258–263, 2007.

12- S. Ansari. Amin, M. Pazouki, and

A.Hosseinnia” Synthesis of TiO2–Ag

Nanocomposite with Sol–Gel Method and

Investigation of its Antibacterial Activity

Against E. Coli”, Powder Technology, Vol.

196, pp. 241–245, 2009.

13- H.E. Chaoa, Y.U. Yuna, H.U. Xingfanga,

and A. Larbot,”E­ect of Silver Doping on the

Phase Transformation and Grain Growth of

Sol-Gel Titania Powder”, Journal of the

European Ceramic Society, Vol. 23, pp. 1457–

1464, 2003.

14- N. Sobana, M. Muruganadham, and M.

Swaminathan,”Nano-Ag particles Doped TiO2

for Efficient Photodegradation of Direct azo

dyes”, Journal of Molecular Catalysis, Vol.

258, pp. 124 –132, 2006.

15- C. Suwanchawalit, P. Chanhom1, P.

Sriprang, and S. Wongnawa” A Ag-Doped

TiO2 Photocatalyst for Dye Decolorization

under UV and Visible Irradiation”, Pure and

Applied Chemistry International Conference,

2011.

16- S. Pal, Y.K. Tak, and J.M. Song, "Does the

Antibacterial Activity of Silver Nanoparticles

Depend on the Shape of the Nanoparticle?" ,

Applied and Enviromental Microbiology, Vol.

73, pp. 1712-1720, 2007.

17- A.A., Ashkarran”Antibacterial Properties

of Silver-Doped TiO2 Nanoparticles Under

Solar Simulated light”, Journal of Theoretical

and Applied Physics,Vol. 4, pp. 1-8, 2011.

18- X. Chen, H.J. Schluesener, " Nanosilv er:

A Nanoproduct in Medical Application",

Toxicology Letters, Vol. 176, p.p 1–12, 2008.

19- R. Mahendra, Y. Alka, and G. Aniket”

Silver Nanoparticles as a New Generation of

Antimicrobials”, Biotechnology Advances,

Vol. 27, pp. 76–83, 2009.

20- L.F. Espinosa-Cristóbal, G.A. MartínezCastañón,R.E. Martínez-Martínez, J.P. LoyolaRodríguez,N. Patiño-Marín, J.F. ReyesMacías,Facundo Ruiz, "Antibacterial Effect of

Silver nanoparticles Against Streptococcus

mutans", Materials Letters, Vol. 63, p.p 2603–

2606,2009.

21- I. Wiegand, K. Hilpert, R. E. W. Hancock,

"Agar and Broth Dilution Methods to

Determine the Minimal Inhibitory

Concentration (MIC) of Antimicrobial

Substances", Nature Protocols, Vol.3,No.2, p.p

163-175, 2008.

22- A. Alem, H. Sarpoolaky, and M.

Keshmiri,” Titania Ultrafiltration membrane:

Preparation, Characterization and

Photocatalytic Activity”, Journal of the

European Ceramic Society, Vol. 29, pp. 629–

635, 2009.

23- C.R. Mahon, D.C. Lehman, G. Manuselis,

Textbook of Diagnostic Microbiology, ISBN:

978-1-4160-6165-6, p. 8-10, 2010.

24- K. Tomono, E. Takigawa, T.Suzuki, and

1. Ohira,” Effecta of TiO2 Photocatalyst on

Physical Properties and Antibacterial Action of

Glass Ionomer Cement for Luting”, Material

Technology, Vol. 22, pp. 83-89, 2004.

25- C. Sahoo, A.K. Gupta and A. Pal,

”Photocatalytic Degradation of Methyl Red

dye in Aqueous Solutions Under UV

Irradiation Using Ag+

 Doped TiO2”,

Desalination, Vol. 181, pp. 91-100, 2005.

26- D.B. Hamal, and K.J. Klabunde,

“Synthesis, Characterization, and Visible Light

Activity of New Nanoparticle Photocatalysts

Based on Silver, Carbon, and Sulfur-Doped

TiO2”, Journal of Colloid and Interface

Science, Vol. 311, pp. 514–522, 2007.