Impacts of Drying Methods on Physical Properties and Release Kinetics of Complex Coacervated Berberine Microcapsules

Subject Areas : food science

maryam keshtkaran ¹ , Maryam Mizani ² , Mohammad Ali Ebrahimzadeh Mousavi ³ , Mohammad Amin Mohammadifar ⁴ , Reza Azizinejad ⁵

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3 - استاد دانشگاه تهران
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Received: 2024-09-07 Accepted : 2024-11-15 Published : 2024-12-28

Keywords: Berberis vulgaris, Berberine, Complex coacervation, Astragalus rahensis, Spray-drying, Freeze-drying.,

Abstract :

Berberine is a multifunctional compound belonging to the Berberidaceae family may be used to promote the quality of industrial food products in the form of dried microcapsules. In this research, the effects of the drying techniques on the coacervated microcapsules as well as the core material's quality and stability were investigated. Berberine microcapsules using Astragalus rahensis/gelatin cross-linked by transglutaminase were dried by two different methods The particle size and polydispersity, efficiency, berberine release kinetics were analyzed. Spray-dried (SD) microcapsules were 37.6–49.6 nm in diameter, whereas freeze-drying produces bigger particles with wider size dispersion. The positive interaction between the low-grade flaky tragacanth and gelatin reduces the undesirable stickiness of SD microcapsules and causes higher drying yields by up to 50 %. The porous structure of the freeze-dried (FD) microcapsules may expose sensitive berberine to oxidative reactions. Differential scanning calorimetry thermograms indicated greater thermal stability of SD microcapsules than FD microcapsules. The release profiles of berberine showed a good fit to the modified Korsmeyer-Peppas model, where the SD microcapsules with the greatest burst effect at 5˚ and 80˚C provide the desirable controlled release characteristics. Berberine SD microcapsules may be used as a safe and nutritious powder in heat-treated food formulations.

References:

Admassu, S., & Kebede, M. (2019). Application of antioxidants in food processing industry: Options to improve the extraction yields and market value of natural products. Advances in Food Technology and Nutritional Sciences, 5(2), 38-49. https://dx.doi.org/ 10.17140/AFTNSOJ-5-155
Alemardan, A., Asadi, W., Rezaei, M., Tabrizi, L., Mohammadi, S. (2013). Cultivation of Iranian seedless barberry (Berberis integerrima ’Bidaneh’):A medicinal shrub. Industrial Crops and Products, 50:276–287. https://doi.org/10.1016/j.indcrop.2013.07.061
Alexandre, J. B., Barroso, T.L.C.T., Oliveira, M.A., Mendes, F.R.S., Costa, J.M.C., R.A. Moreira, R.A., Furtado, R.F. (2019). Cross-linked coacervates of cashew gum and gelatin in the encapsulation of pequi oil. Ciência Rural, 49 (12), 1-12. https://doi.org/10.1590/01038478cr20190079
Alvim, I. D., & Grosso, C. R. F. (2010). Microparticles obtained by complex coacervation: influence of the type of reticulation and the drying process on the release of the core material. Food Science and Technology, 30(4), 1069-1076. https://doi.org/10.1590/S0101-20612010000400036
Anwar, S. H., Kunz, B. (2011). The influence of drying methods on the stabilization of fish oil microcapsules: comparison of spray granulation, spray drying and freeze drying. Journal of Food Engineering, 105 (2), 367-378. https://doi.org/10.1016/j.jfoodeng.2011.02.047
Baracat, M. M., Nakagawa, A. M., Casagrande, R., Georgetti, S. R., Verri, W. A., & de Freitas, O. (2012). Preparation and characterization of microcapsules based on biodegradable polymers: pectin/casein complex for controlled drug release systems. AAPS PharmSciTech, 13(2), 364-372. https://doi.org/10.1208/s12249-012-9752-0
Bhat, R., Paliyath, G. (2015). Fruits of Tropical Climates: Biodiversity and Dietary Importance. In Encyclopedia of Food and Health, 3, 00337, 138–143. http://dx.doi.org/10.1016/B978-0-12-384947-2.00337-8
Bi, H., Xu, Y., Fan, F., & Sun, X. (2022). Effect of drying methods on Lactobacillus Rhamnosus GG microcapsules prepared using the complex coacervation method. Journal of Food Science, 87(3), 1282-1291. https://doi.org/10.1111/1750-3841.16061
Bohidar, H. B., & Jena, S. S. (1993). Kinetics of sol–gel transition in thermoreversible gelation of gelatin. The Journal of Chemical Physics, 98(11), 8970-8977. https://doi.org/10.1063/1.464456
Carpentier, J., Conforto, E., Chaigneau, C., Vendeville, J-E., Maugard, Th. (2022). Microencapsulation and controlled release of α-tocopherol by complex coacervation between pea protein and tragacanth gum: A comparative study with arabic and tara gums. Innovative Food Science and Emerging Technologies, 77, 102951,1-14. https://doi.org/10.1016/j.ifset.2022.102951
Carra, J. B., de Matos, R. L. N., Ana Paula Novelli, A. P., do Couto, R. O., Yamashita, F., dos Santos Ribeiro, M. A., Meurer, E. C., Junior, W. A. V., Casagrande, R., Georgetti, S. R., Arakawa, N. S., Baracat, M. M. (2022). Spray-drying of casein/pectin bioconjugate microcapsules containing grape (Vitis labrusca) by-product extract. Food Chemistry. 368(130817), 1-10. https://doi.org/10.1016/j.foodchem.2021.130817
Chegini, G., & Ghobadian, B. (2007). Spray dryer parameters for fruit juice drying. World Journal of Agricultural Sciences, 3(2), 230-236.
Chen, C., Tao, C., Liu, Z., Lu, M., Pan, Q., Zheng, Li, Q., Song, Zh., Fichna, J. (2015). A randomized clinical trial of berberine hydrochloride in patients with diarrhea‐predominant irritable bowel syndrome. Phytotherapy Research, 29(11), 1822-1827. https://doi.org/10.1002/ptr.5475
Comunian, T. A., Gomez-Estaca, J., Ferro-Furtado, R., Conceição, G. J. A., Moraes, I. C. F., de Castro, I. A., & Favaro-Trindade, C. S. (2016). Effect of different polysaccharides and crosslinkers on echium oil microcapsules. Carbohydrate Polymers, 150, 319-329. https://doi.org/10.1016/j.carbpol.2016.05.044
Cruz, M.C.R., Dagostin, J.L.A., Perussello, C.A., Masson M.L. (2019). Assessment of physicochemical characteristics, thermal stability and release profile of ascorbic acid microcapsules obtained by complex coacervation. Food Hydrocolloids, 87, 71-82 https://doi.org/10.1016/j.foodhyd.2018.07.043
Cui, T., Chen, C., Jia, A., Li, D., Shi, Y., Zhang, M., Bai, X., Liu, X. (2021). Changheng Liu a,bCharacterization and human microfold cell assay of fish oil microcapsules: Effect of spray drying and freeze-drying using konjac glucomannan (KGM)-soybean protein isolate (SPI) as wall materials. Journal of Functional Foods. 83, 104542, 1-9. https://doi.org/10.1016/j.jff.2021.104542
Delassus, P. (1997). Barrier polymers. In The Wiley Encyclopedia of Packaging Technology, 71–77. https://doi.org/10.1002/0471238961.0201181804051201.a01.pub2
De Oliveira, E. F., Paula, H. C., & de Paula, R. C. (2014). Alginate/cashew gum nanoparticles for essential oil encapsulation. Colloids and Surfaces B: Biointerfaces, 113, 146-151. https://doi.org/10.1016/j.colsurfb.2013.08.038
Dianawati, D., Mishra, V., & Shah, N. P. (2013). Effect of drying methods of microencapsulated Lactobacillus acidophilus and Lactococcus lactis ssp. cremoris on secondary protein structure and glass transition temperature as studied by Fourier transform infrared and differential scanning calorimetry. Journal of Dairy Science, 96(3), 1419-1430. https://doi.org/10.3168/jds.2012-6058
Dong, Z., Ma, Y., Hayat, K., Jia, C., Xia, S., & Zhang, X. (2011). Morphology and release profile of microcapsules encapsulating peppermint oil by complex coacervation. Journal of Food Engineering, 104(3), 455-460. https://doi.org/10.1016/j.jfoodeng.2011.01.011
Durrieu, V., Gandini, A.(2005). Preparation of aqueous anionic poly(urethane-urea) dispersions. Influence of the incorporation of acrylic, polycarbonate and perfluoro-oligoether diols on the dispersion and polymer properties. Polymer Advanced Technology, 16:840–845. https://doi.org/10.1002/pat.667
Eratte, D., Wang, B., Dowling, K., Barrow, C. J., & Adhikari, B. P. (2014). Complex coacervation with whey protein isolate and gum arabic for the microencapsulation of omega-3 rich tuna oil. Food & Function, 5(11), 2743-2750. https://doi: 10.1039/c4fo00296b.
Ezhilarasia, P. N., Indranib, D., Jenac, B.S. Anandharamakrishnan, C. (2013). Freeze drying technique for microencapsulation of Garcinia fruit extract and its effect on bread quality. Journal of Food Engineering.117(4), 513-520. https://doi.org/10.1016/j.jfoodeng.2013.01.009
Fernandes, R. V. d. B., Borges, S. V., & Botrel, D. A. (2013). Influence of spray drying operating conditions on microencapsulated rosemary essential oil properties. Food Science and Technology, 33, 171-178. https://doi.org/10.1590/S0101-20612013000500025

Frascareli, E., Silva, V., Tonon, R., & Hubinger, M. (2012). Effect of process conditions on the microencapsulation of coffee oil by spray drying. Food and Bioproducts Processing, 90(3), 413-424. https://doi.org/10.1016/j.fbp.2011.12.002
Fredlake, C. p., Crosthwaite, J. Hert, M., D. G., Aki S. N. V. K., and Brennecke, J. F. (2004). Thermophysical Properties of Imidazolium-Based Ionic Liquids. Journal of Chemical & Engineering Data, 49, 954–964. https://doi.org/10.1021/je034261a
Furlán, L. T. R., Lecot, J., AntonioPérez Padilla, A. P., Campderrós, M. E., Zaritzky, N. (2011). Effect of saccharides on glass transition temperatures of frozen and freeze dried bovine plasma protein. Journal of Food Engineering, 106(1), 74-79. https://doi.org/10.1016/j.jfoodeng.2011.04.010
Ghosi, Gh. (2021).The beginning of harvesting seedless barberry from the southern Khorasan; Medicinal Plants Educational- News Network, www.medplant.ir, [in Persian].
Glomm, W. R., Molesworth, P. P., Sandru, E. M., Truong, L. T., Brunsvik, A., & Johnsen, H. (2021). Microencapsulation of Peppermint Oil by Complex Coacervation and Subsequent Spray Drying Using Bovine Serum Albumin/Gum Acacia and an Oxidized Starch Crosslinker. Applied Sciences, 11(9), 3956. https://doi.org/10.3390/app11093956
Górska, A., Szulc, K., Ostrowska-Ligęza, E., Bryś, J., Wirkowska-Wojdyła, M. (2017). Effect of composition and drying method on glass transition temperature, water sorption characteristics and surface morphology of newly designed β-lactoglobulin/retinyl palmitate/disaccharides systems. Journal of Thermal Analysis Colorimetery, 130(1):177–85. https://doi.org/10.1007/s10973-017-6392-3
Gouin, S. (2004). Microencapsulation: industrial appraisal of existing technologies and trends. Trends in Food Science & Technology, 15(7-8), 330-347. https://doi.org/10.1016/j.tifs.2003.10.005
Hatch, L. (2007).Cultivars of Woody Plants, Volume 1: A-G, TCR Press.
Ho, L. Y., Lim, Y. Y., Tan, C. P., & Siow, L. F. (2022). Effects of spray-, oven-, and freeze drying on the physicochemical properties of poorly aqueous-soluble xanthone encapsulated by coacervation: A comparative study. Drying Technology, 40(3), 505-515. https://doi.org/10.1080/07373937.2020.1810697
Holkem, A. T., Raddatz, G. C., Nunes, G. L., Cichoski, A. J., Jacob-Lopes, E., Grosso, C. R. F., & de Menezes, C. R. (2016). Development and characterization of alginate microcapsules containing Bifidobacterium BB-12 produced by emulsification/internal gelation followed by freeze drying. LWT-Food Science and Technology, 71, 302-308. http://dx.doi.org/10.1016%2Fj.lwt.2016.04.012
Hu, X., Li, Y., Zhang, E., Wang, X., Xing, M., Wang, Q., Lei, J., Huang, H. (2012) Preparation and evaluation of orally disintegrating tablets containing taste-masked microcapsules of berberine hydrochloride. AAPS PharmSciTech, 14(1):29–37. https://doi.org/10.1208/s12249-012-9880-6
Huang, X., & Brazel, C. S. (2001). On the importance and mechanisms of burst release in matrix-controlled drug delivery systems. Journal of Controlled Release, 73(2-3), 121-136. https://doi.org/10.1016/s0168-3659(01)00248-6
Ikuta, A., & Itokawa, H. (1988). Berberine: production through plant (Thalictrum spp.) cell cultures. Medicinal and Aromatic Plants I, 282-293. https://doi.org/10.1007/978-3-642-73026-9_15
Jain, A., Thakur, D., Ghoshal, G., Katare, O., & Shivhare, U. (2015). Microencapsulation by complex coacervation using whey protein isolates and gum acacia: an approach to preserve the functionality and controlled release of β-carotene. Food and Bioprocess Technology, 8(8), 1635-1644. http://dx.doi.org/10.1007%2Fs11947-015-1521-0
Jain, A., Thakur, D., Ghoshal, G., Katare, O., & Shivhare, U. (2016). Characterization of microcapsulated β-carotene formed by complex coacervation using casein and gum tragacanth. International Journal of BIological Macromolecules, 87, 101-113. https://doi.org/10.1016/j.ijbiomac.2016.01.117
Jin, R., Yin, H., Wang, H., Zhang, D., Cao, K., & Yuan, C. (2018). Preparation and characterization of squalene microcapsules by complex coacervation. Journal of Food Process Engineering, 41(6), e12847, 1-6. https://doi.org/10.1111/jfpe.12847
Jovanovi´c, A. A., Levi´c, S. M., Pavlovic, V. B., Markovic, S. B., Pjanovic, R. V., Ðordevi´c, V. B., Nedovi´c, V., Bugarski, B. M. (2021). Freeze versus spray drying for dry wild thyme (Thymus serpyllum L.) extract formulations: The impact of gelatin as a coating material. Molecules, 26 (13), 3933, 1-16. http://dx.doi.org/10.5937/leksir2141041J
Kanha, N.; Surawang, S.; Pitchakarn, P.; Laokuldilok, T. (2020). Microencapsulation of copigmented anthocyanins using double emulsion followed by complex coacervation: Preparation, characterization and stability. LWT, 133, 110154. http://dx.doi.org/10.1016/j.lwt.2020.110154.

Kanha, N., Regenstein, J. M., Surawang, S., Pitchakarn, P., & Laokuldilok, T. (2021). Properties and kinetics of the in vitro release of anthocyanin-rich microcapsules produced through spray and freeze-drying complex coacervated double emulsions. Food Chemistry, 340, 127950, 1-11. https://doi.org/10.1016/j.foodchem.2020.127950
Karsa DR, Stephenson RA. (1993). Encapsulation and Controlled Release. (2nd ed.). England.
Kaushik, P., Dowling, K., McKnight, S., Barrow, C. J., & Adhikari, B. (2016). Microencapsulation of flaxseed oil in flaxseed protein and flaxseed gum complex coacervates. Food Research International, 86, 1-8. http://dx.doi.org/10.1016/j.foodres.2016.05.015
Keshtkaran, M., Mizani, M., Mousavi, S. M. E., Mohammadifar, M. A., & Azizinejad, R. (2022). Extraction optimization and microencapsulation of Berberine from Berberis vulgaris incorporated in a functional orange drink: Physiochemical attributes and kinetic release studies. Journal of Berry Research, 12, 93-113. http://doi.org/10.3233/jbr-211504
Kirk, R. E., Othmer, D. F., Grayson, M., Eckroth, D., et al. (2004). Kirk-Othmer Encyclopedia of Chemical Technology. Wiley. 4th edition. 486.
Korsmeyer, R. W., Gurny, R., Doelker, E., Buri, P., & Peppas, N. A. (1983). Mechanisms of solute release from porous hydrophilic polymers. International Journal of Pharmaceutics, 15(1), 25-35. https://doi.org/10.1016/0378-5173(83)90064-9
Lam, P. L., Lee, K. K. H., Kok, S. H. L., Cheng, G. Y. M., Tao, X. M., Hao, D. K. P., Yuen, M. C. W., Lam, K. H., Gambari, R., Chui, C. H., Wong, R. S. M. (2012). Development of formaldehyde-free agar/gelatin microcapsules containing Berberine HCl and gallic Acid and their topical and oral applications. Soft Matter, 8, 5027–5037. https://doi.org/10.1039/C2SM07236J
Li, Z., Geng, Y-N., Jiang, J-D., & Kong, W-J. (2014). Antioxidant and anti-inflammatory activities of berberine in the treatment of diabetes mellitus. Evidence-Based Complementary and Alternative Medicine, 2014,1-13. https://doi.org/10.1155/2014/289264
Li, D., Liu, B., Yang, F., Wang, X., Shen, H., & Wu, D. (2016). Preparation of uniform starch microcapsules by premix membrane emulsion for controlled release of avermectin. Carbohydrate polymers, 136, 341-349. https://doi.org/10.1016/j.carbpol.2015.09.050

Liu, Y., Zhang, L., Song, H., & Ji, G. (2013). Update on berberine in nonalcoholic fatty liver disease. Evidence-Based Complementary and Alternative Medicine, 1-8. https://doi.org/10.1155/2013/308134
Lu, Y., Zhang, B., Shen, H., Ge, X., Sun, X., Zhang, Q., Zhang, X., Sun, Z., Li, W. (2022). Sodium caseinate and acetylated mung bean starch for the encapsulation of lutein: enhanced solubility and stability of lutein. Foods, 11, 65, 1-19. https://doi.org/10.3390/foods11010065
Martín-Alfonso, J., Číková, E., & Omastová, M. (2019). Development and characterization of composite fibers based on tragacanth gum and polyvinylpyrrolidone. Composites Part B: Engineering, 169, 79-87. https://doi.org/10.1016/j.compositesb.2019.04.005
Mirhosseini, H., Amid, B. T. (2013). Effect of different drying techniques on flowability characteristics and chemical properties of natural carbohydrate-protein gum from durian fruit seed. Chemistry Central Journal, 7(1): 1-14. https://doi.org/10.1186%2F1752-153X-7-1
Molaahmadi Bahraseman, N., Shekarchizadeh, H., Goli, S. A. H. (2022). Thermodynamic compatibility of gelatin and tragacanth gum in aqueous systems. Food Chemistry, 373, 131584 1-11. https://doi.org/10.1016/j.foodchem.2021.131584
Nogueira, M. B., Prestes, C. F., & Burkert, J. F. d. M. (2017). Microencapsulation by lyophilization of carotenoids produced by Phaffia rhodozyma with soy protein as the encapsulating agent. Food Science and Technology, 37, 1-4. https://doi.org/10.1590/1678-457X.05417
Ozkan, G., Franco, P., De Marco, I., Xiao, J., & Capanoglu, E. (2019). A review of microencapsulation methods for food antioxidants: Principles, advantages, drawbacks and applications. Food Chemistry, 272, 494-506. https://doi.org/10.1016/j.foodchem.2018.07.205
Pang, Y., Duan, X., Ren, G., & Liu, W. (2017). Comparative study on different drying methods of fish oil microcapsules. Journal of Food Quality, 2017, 1-7. https://doi.org/10.1155/2017/1612708.
Pudziuvelyte, L., Marksa, M., Sosnowska, K., Winnicka, K., Morkuniene, R., & Bernatoniene, J. (2020). Freeze-drying technique for microencapsulation of Elsholtzia ciliata ethanolic extract using different coating materials. Molecules, 25(9), 2237, 1-16. https://doi.org/10.3390/molecules25092237
Pui, L. Ph., Saleena, L. A. K. (2022). Effects of spray-drying parameters on physicochemical properties of powdered fruits. Foods and Raw Materials, 10(2), 235–251. https://doi.org/10.21603/2308-4057-2022-2-533
Pulicharla, R., Marques, C., Das, R. K., Rouissi, T., & Brar, S. K. (2016). Encapsulation and release studies of strawberry polyphenols in biodegradable chitosan nanoformulation. International Journal of Biological Macromolecules, 88, 171-178. https://doi.org/10.1016/j.ijbiomac.2016.03.036
Qi, X., Lan, Y., Ohm, J.-B., Chen, B., & Rao, J. (2021). The viability of complex coacervate encapsulated probiotics during simulated sequential gastrointestinal digestion affected by wall materials and drying methods. Food & Function, 12(19), 8907-8919. https://doi.org/10.1039/D1FO01533H
Quispe-Condori, S., Saldaña, M. D., & Temelli, F. (2011). Microencapsulation of flax oil with zein using spray and freeze drying. LWT-Food Science and Technology, 44(9), 1880-1887. https://doi.org/10.1016/j.lwt.2011.01.005
Ritger, P. L., & Peppas, N. A. (1987). A simple equation for description of solute release II. Fickian and anomalous release from swellable devices. Journal of Controlled Release, 5(1), 37-42. https://doi.org/10.1016/0168-3659(87)90035-6
Rocha, G. A., Fávaro-Trindade, C. S., & Grosso, C. R. F. (2012). Microencapsulation of lycopene by spray drying: characterization, stability and application of microcapsules. Food and Bioproducts Processing, 90(1), 37-42. https://doi.org/10.1016/j.fbp.2011.01.001
Rojas-Moreno, S., Cárdenas-Bailón, F., Osorio-Revilla, G., Gallardo-Velázquez, T., & Proal-Nájera, J. (2018). Effects of complex coacervation-spray drying and conventional spray drying on the quality of microencapsulated orange essential oil. Journal of Food Measurement and Characterization, 12(1), 650-660. https://doi.org/10.1007/s11694-017-9678-z
Rokade, M., Vichare, V., Neve, T., Parande, BH., Dhole, SH. (2022). A review on anticancer potential of Berberis aristata and Berberine with focus on quantitative methods. Journal of Preventive, Diagnostic and Treatment Strategies in Medicine, 1(2), 67-75. https://doi.org/ 10.4103/jpdtsm.jpdtsm_9_22
Saffari, M. M., Farzi, M., Emam‐Djomeh, Z., Moini, S., & Mohammadifar, M. A. (2013). Applying iranian gum tragacanth to improve textural properties of maltodextrin microcapsules. Journal of Texture Studies, 44(1), 12-20. https://doi.org/10.1111/j.1745-4603.2012.00359.x
Schmeller, T., Latz-Brüning, B., & Wink, M. (1997). Biochemical activities of berberine, palmatine and sanguinarine mediating chemical defence against microorganisms and herbivores. Phytochemistry, 44(2), 257-266. https://doi.org/10.1016/S0031-9422(96)00545-6
Seregelj, V., Cetkovic, G., Canadanovic-Brunet, J., Saponjac, V.T., Vulic, J., Stajcic, S. (2020). Encapsulation and Degradation Kinetics of Bioactive Compounds from Sweet Potato Peel During Storage. Food Technology & Biotechnology, 58, 314-324. https://doi.org/10.17113/ftb.58.03.20.6557
Sheikh, F. A., Aamir, M. N., Shah, M. A., Ali, L., Anwer, K., & Javaid, Z. (2020). Formulation design, characterization and in vitro drug release study of orodispersible film comprising BCS class II drugs. Pakistan Journal of Pharmaceutical Sciences, 33, 343-353. https://doi.org/10.36721/PJPS.2020.33.1.SUP.343-353.1
Singh, S. S., Aswal, V., & Bohidar, H. (2007). Structural studies of agar–gelatin complex coacervates by small angle neutron scattering, rheology and differential scanning calorimetry. International Journal of Biological Macromolecules, 41(3), 301-307. https://doi.org/10.1016/j.ijbiomac.2007.03.009
Wang, X., Lee, J., Wang, Y-W., & Huang, Q. (2007). Composition and rheological properties of β-lactoglobulin/pectin coacervates: effects of salt concentration and initial protein/polysaccharide ratio. Biomacromolecules, 8(3), 992-997. https://doi.org/10.1021/bm060902d
Wang, Y., Liu, Y., Du, X., Ma, H., Yao, J. (2020). The anti-cancer mechanisms of berberine: a review. Cancer Management and Research. 12, 695–702. https://doi.org/10.2147/CMAR.S242329
Warowicka, A., Nawrot, R., Goździcka-Józefiak, A. (2020). Antiviral activity of berberine. Advances in Virology, 165(9):1935–1945. https://doi.org/10.1007/s00705-020-04706-3
Wardhani, D. H., Wardana, I. N., Ulya, H. N., Cahyono, H., Kumoro, A. C., & Aryanti, N. (2020). The effect of spray-drying inlet conditions on iron encapsulation using hydrolysed glucomannan as a matrix. Food and Bioproducts Processing, 123, 72–79. https://doi.org/10.1016/j.fbp.2020.05.013
Wu, J., Yu, D., Sun, H., Zhang, Y., Zhang, W., Meng, F., & Du, X. (2015). Optimizing the extraction of anti-tumor alkaloids from the stem of Berberis amurensis by response surface methodology. Industrial Crops and Products, 69, 68-75. https://doi.org/10.1016/j.indcrop.2015.01.053
Xia, L. M., & Luo, M. H. (2015). Study progress of berberine for treating cardiovascular disease. Chronic Diseases and Translational Medicine 1, 231–235. https://doi.org/10.1016/j.cdtm.2015.11.006
Xiao, J., Nian, S., & Huang, Q. (2015). Assembly of kafirin/carboxymethyl chitosan nanoparticles to enhance the cellular uptake of curcumin. Food Hydrocolloids, 51, 166-175. https://doi.org/10.1016/j.foodhyd.2015.05.012
Yeung, A.W.K., Orhan, I.E., Aggarwal, B.B., Battino, M., Belwal, T., Bishayee, A., Daglia, M., Devkota, H.P., El-Demerdash, A., Balacheva, A.A., et al. (2020). Berberine, a popular dietary supplement for human and animal health: Quantitative research literature analysis—A review. Animal Science Papers and Reports. 38, 5–19 https://doi.org/10.1039/c2sm07236j
Yu, F., Li, Z., Zhang, T., Wei, Y., Xue, Y., & Xue, C. (2017). Influence of encapsulation techniques on the structure, physical properties, and thermal stability of fish oil microcapsules by spray drying. Journal of Food Process Engineering, 40(6), e12576, 1-9. https://doi.org/10.1111/jfpe.12576
Zajic, J., Panchal, C., & Westlake, D. (1976). Bio-emulsifiers. CRC Critical Reviews in Microbiology, 5(1), 39-66. https://doi.org/10.3109/10408417609102309
Zhao, Y., Khalid, N., Shu, G., Neves, M. A., Kobayashi, I., & Nakajima, M. (2019). Complex coacervates from gelatin and octenyl succinic anhydride modified kudzu starch: Insights of formulation and characterization. Food Hydrocolloids, 86, 70–77. http://doi.org/10.1016/j.foodhyd.2018.01.040
Zhao, J. V., Yeung, W. F., Chan, Y. H., Vackova, D., Leung, J.Y.Y., Ip, D.K. M., et al. (2021). Effect of Berberine on Cardiovascular Disease Risk Factors: A Mechanistic Randomized Controlled Trial. Nutrients, 13(8), 2550, 1-10. https://doi.org/10.3390/nu13082550
Zhang, K., Yao, X., Gao, Y., Liu, N., Qiu, J., Li, Sh., Liu, X., He, Y. (2021). Improvement on storage stability of iron-fortified soybean powder by microencapsulation. Journal of Food Science, 86(3): 996-1003. https://doi.org/10.1111/1750-3841.15621
Zuidam, N., Nedovic, V. (2010). Encapsulation Technologies for Active Food Ingredients and Food Processing. Springer Science+Business Media. LLC.