Radiological and histopathological study of the effects of Kakuti plant (Ziziphora tenuior) on bone defect repair in rabbit animal model

Subject Areas : Radiology and Ultrasound

Siamak Alizadeh ¹ , Soha Rezazadeh ²

1 - Department of Veterinary Medicine, Nag.C., Islamic Azad University, Naghadeh, Iran
2 - Faculty of Veterinary Medicine, Ur.C., Islamic Azad University, Urmia, Iran

Received: 2024-10-13 Accepted : 2025-02-15 Published : 2025-10-25

Keywords: Bone defect, Histopathology, Kakuti plant (Ziziphora tenuior), Rabbit, Radiology,

Abstract :

 This study aimed to assess the radiological and histopathological effects of the hydroalcoholic extract of the Kakuti (Ziziphora tenuior) plant on bone healing in a rabbit model. The current study involved 10 male New Zealand white rabbits, aged 12–18 months with a mean weight of 1.1-1.7 kg. The rabbits were randomly divided into two groups: a control group and a treatment group. The rabbits were anesthetized via intramuscular injection of a mixture containing xylazine and ketamine; then, a 3 mm diameter and depth defect was created in the radius bone. In the sample group (n=5), 1 g of Kakuti extract was placed inside the defect cavity, and the skin and muscles were sutured as usual. Both groups were evaluated radiologically and histopathologically at two- and seven-weeks post-surgery. Results showed no significant difference between the two groups in callus formation, healing, or remodeling on radiographs at these time points (p> 0.05). Histopathological findings indicated no significant difference in connective tissue formation between the control and treatment groups at either time point, while bone tissue formation showed a significant difference (p> 0.05). These findings suggest that Ziziphora tenuior extract accelerates bone healing in the rabbit radius but does not significantly influence cartilage healing.

 

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