Comparable Antibacterial Effects of Zinc and graphene Oxide Nanoparticles on Urinary Calculi obtained after Percutaneous Nephrolithotomy
Subject Areas : Medical MicrobiologyZeinab Piravar 1 , Mehrdad Jafari Fesharaki 2 , Hedieh Assar 3
1 - Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2 - 2. Department of Cardiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 - Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Keywords: Graphene Oxide, Zinc Oxide, nanoparticles, urolithiasis, Bacterial infection,
Abstract :
Background and Objective: Kidney stone disease and its associated infections have become a public health concern. Given the problem of bacterial resistance, the production of novel antimicrobial agents has always been of interest. Graphene oxide and zinc oxide nanoparticles have drawn the attention of researchers in the treatment of bacterial infections due to their antimicrobial properties. In this study, the antibacterial effects of zinc oxide and graphene oxide nanoparticles on bacterial infections caused by kidney stones isolated by nephrolithotomy were investigated.
Materials and Methods: Kidney stones of 45 patients were removed from their kidneys by Percutaneous nephrolithotomy surgery at Shahid Labafi Nejad Hospital in Tehran. After culturing and identifying the bacteria in the stones, the antibacterial activity of ZnO and GO nanoparticles was investigated by determining the minimum inhibitory concentration and disk diffusion method.
Results: FeSEM, Zeta test and DLS of the nanoparticles showed the purity of the synthesized nanoparticles. The antibacterial effects of zinc oxide nanoparticles at a concentration of 1000 ppm showed maximum inhibitory effect and a 25 mm zone of inhibition, and graphene oxide at the same concentration and 18 mm zone of inhibition was obtained for Escherichia coli bacteria. The MBC results of zinc oxide nanoparticle were 228 µg/ml and graphene oxide 300 µg/ml and MIC of zinc oxide nanoparticles 180 µg/ml and graphene oxide 250 µg/ml were obtained.
Conclusion: The synthesized zinc oxide and graphene oxide nanoparticles have antibacterial effects on the isolated bacteria and can be used in the treatment of infections caused by kidney stones.
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