The effect of six weeks of moderate-intensity aerobic training with cannabis supplementation on prostate PI3 gene expression in male rat fed with high-fat diet

Subject Areas : Open Access

Marzieh Hafezi eirdmousa ¹ , mandana gholami ² , Heshmatolah Parsian ³

1 -
2 - Assistant professor, department of physical education and sport sciences, science and research branch, Islamic azad university, Tehran, Iran
3 - Department of Physical Education and Sport Sciences, Shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

Received: 2025-01-23 Accepted : 2025-02-11 Published : 2024-09-18

Keywords: Moderate-intensity aerobic exercise, cannabis, prostate, high-fat diet, PI3 gene,

Abstract :

Introduction: Epidemiological and clinical evidence consistently links obesity to accelerated prostate cancer progression and increased mortality. However, the physiological mechanisms associated with obesity and prostate cancer markers remain unclear. Over the past decade, numerous epidemiological studies have shown that obesity is associated with increased risk and mortality from a variety of cancers, including prostate cancer.

Methods: Male Wistar rats weighing 240±20 g were used in this study. Rats were randomly divided into five groups: normal control, high-fat control, exercise, supplementation, exercise, and supplementation. Cannabis extract was administered at a dose of 100 mg/kg/day for 5 days per week for 6 weeks via gavage. The exercise program consisted of treadmill running at a minimum speed of 15 m/min for 30 min and a maximum speed of 27 m/min for 60 min per day, 5 days per week for 8 weeks. Biopsy was performed 48 hours after the end of the intervention.

Findings: Based on the results obtained from the two-way analysis of variance test, it was determined that aerobic exercise had a significant effect on the expression of the PI3 gene in prostate tissue (P=0.015). Cannabis supplementation also had a significant effect on the expression of the PI3 gene in prostate tissue (P=0.004). However, the interaction of aerobic exercise and cannabis supplementation had a significant effect on the expression of the PI3 gene in prostate tissue (P=0.068).

Conclusion: It seems that aerobic exercise along with the use of cannabis supplementation can modulate the level of the PI3 gene, which indicates the anti-inflammatory effects of cannabis and its possible role in preventing prostate cancer.

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